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Effects of age on lacrimal gland bioactive lipids.
Ebright, Brandon; Yu, Zhiyuan; Dave, Priyal; Dikeman, Dante; Hamm-Alvarez, Sarah; de Paiva, Cintia S; Louie, Stan.
Afiliación
  • Ebright B; Department of Clinical Pharmacy, Alfred Mann School of Pharmacy and Pharmaceutical Sciences, University of Southern California, Los Angeles, CA, United States, 90089-9121, USA. Electronic address: bebright@usc.edu.
  • Yu Z; Department of Ophthalmology, Baylor College of Medicine, Houston, TX, USA. Electronic address: zhiyuanyu@hotmail.com.
  • Dave P; Department of Clinical Pharmacy, Alfred Mann School of Pharmacy and Pharmaceutical Sciences, University of Southern California, Los Angeles, CA, United States, 90089-9121, USA. Electronic address: priyalda@usc.edu.
  • Dikeman D; Department of Clinical Pharmacy, Alfred Mann School of Pharmacy and Pharmaceutical Sciences, University of Southern California, Los Angeles, CA, United States, 90089-9121, USA. Electronic address: dikeman@usc.edu.
  • Hamm-Alvarez S; Department of Ophthalmology, Baylor College of Medicine, Houston, TX, USA; Department of Pharmaceutical Sciences, Alfred Mann School of Pharmacy and Pharmaceutical Sciences, University of Southern California, Los Angeles, CA, USA; Department of Ophthalmology, Roski Eye Institute, Keck School of Medi
  • de Paiva CS; Department of Ophthalmology, Baylor College of Medicine, Houston, TX, USA. Electronic address: cintiadp@bcm.edu.
  • Louie S; Department of Clinical Pharmacy, Alfred Mann School of Pharmacy and Pharmaceutical Sciences, University of Southern California, Los Angeles, CA, United States, 90089-9121, USA. Electronic address: slouie@usc.edu.
Ocul Surf ; 33: 64-73, 2024 Jul.
Article en En | MEDLINE | ID: mdl-38705236
ABSTRACT

PURPOSE:

Polyunsaturated fatty acids (PUFA) are a source of bioactive lipids regulating inflammation and its resolution.

METHODS:

Changes in PUFA metabolism were compared between lacrimal glands (LGs) from young and aged C57BL/6 J mice using a targeted lipidomics assay, as was the gene expression of enzymes involved in the metabolism of these lipids.

RESULTS:

Global reduction in PUFAs and their metabolites was observed in aged LGs compared to young controls, averaging between 25 and 66 % across all analytes. ꞷ-6 arachidonic acid (AA) metabolites were all reduced in aged LGs, where the changes in prostaglandin E2 (PGE2) and lipoxin A4 (LXA4) were statistically significant. Several other 5-lipoxygenase (5-LOX) mediated metabolites were significantly reduced in the aged LGs, including D-series resolvins (e.g., RvD4, RvD5, and RvD6). Along with the RvDs, several ꞷ-3 docosahexaenoic acid (DHA) metabolites such as 14-HDHA, neuroprotectin D1 (NPD1), Maresin 2 (MaR2), and MaR 1 metabolite (22-COOH-MaR1) were significantly reduced in aged LGs. Similarly, ꞷ-3 eicosapentaenoic acid (EPA) and its metabolites were significantly reduced in aged LGs, where the most significantly reduced was 18-HEPE. Using metabolite ratios (productprecursor) for specific metabolic conversions as surrogate enzymatic measures, reduced 12-LOX activity was identified in aged LGs.

CONCLUSION:

In this study, global reduction of PUFAs and their metabolites was found in the LGs of aged female C57BL/6 J compared to young controls. A consistent reduction was observed across all detected lipid analytes except for ꞷ-3 docosapentaenoic acid (DPA) and its special pro-resolving mediator (SPM) metabolites in aged mice, suggesting an increased risk for LG inflammation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Envejecimiento / Ácidos Grasos Insaturados / Aparato Lagrimal / Ratones Endogámicos C57BL Límite: Animals Idioma: En Revista: Ocul Surf Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Envejecimiento / Ácidos Grasos Insaturados / Aparato Lagrimal / Ratones Endogámicos C57BL Límite: Animals Idioma: En Revista: Ocul Surf Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos