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The gap gene of Rhizobium etli is required for both free life and symbiosis with common beans.
Casas-Román, Ariana; Lorite, María-José; Werner, Mariana; Muñoz, Socorro; Gallegos, María-Trinidad; Sanjuán, Juan.
Afiliación
  • Casas-Román A; Departmento de Microbiología del Suelo y la Planta, Estación Experimental del Zaidín (CSIC), Granada, Spain.
  • Lorite MJ; Departmento de Microbiología del Suelo y la Planta, Estación Experimental del Zaidín (CSIC), Granada, Spain.
  • Werner M; Departmento de Microbiología del Suelo y la Planta, Estación Experimental del Zaidín (CSIC), Granada, Spain.
  • Muñoz S; Departmento de Microbiología del Suelo y la Planta, Estación Experimental del Zaidín (CSIC), Granada, Spain.
  • Gallegos MT; Departmento de Microbiología del Suelo y la Planta, Estación Experimental del Zaidín (CSIC), Granada, Spain. Electronic address: maritrini.gallegos@eez.csic.es.
  • Sanjuán J; Departmento de Microbiología del Suelo y la Planta, Estación Experimental del Zaidín (CSIC), Granada, Spain. Electronic address: juan.sanjuan@eez.csic.es.
Microbiol Res ; 284: 127737, 2024 Jul.
Article en En | MEDLINE | ID: mdl-38705080
ABSTRACT
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH or Gap) is a ubiquitous enzyme essential for carbon and energy metabolism in most organisms. Despite its primary role in sugar metabolism, GAPDH is recognized for its involvement in diverse cellular processes, being considered a paradigm among multifunctional/moonlighting proteins. Besides its canonical cytoplasmic location, GAPDH has been detected on cell surfaces or as a secreted protein in prokaryotes, yet little is known about its possible roles in plant symbiotic bacteria. Here we report that Rhizobium etli, a nitrogen-fixing symbiont of common beans, carries a single gap gene responsible for both GAPDH glycolytic and gluconeogenic activities. An active Gap protein is required throughout all stages of the symbiosis between R. etli and its host plant Phaseolus vulgaris. Both glycolytic and gluconeogenic Gap metabolic activities likely contribute to bacterial fitness during early and intermediate stages of the interaction, whereas GAPDH gluconeogenic activity seems critical for nodule invasion and nitrogen fixation. Although the R. etli Gap protein is secreted in a c-di-GMP related manner, no involvement of the R. etli gap gene in c-di-GMP related phenotypes, such as flocculation, biofilm formation or EPS production, was observed. Notably, the R. etli gap gene fully complemented a double gap1/gap2 mutant of Pseudomonas syringae for free life growth, albeit only partially in planta, suggesting potential specific roles for each type of Gap protein. Nevertheless, further research is required to unravel additional functions of the R. etli Gap protein beyond its essential metabolic roles.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Simbiosis / Phaseolus / Rhizobium etli Idioma: En Revista: Microbiol Res Asunto de la revista: MICROBIOLOGIA / SAUDE AMBIENTAL Año: 2024 Tipo del documento: Article Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Simbiosis / Phaseolus / Rhizobium etli Idioma: En Revista: Microbiol Res Asunto de la revista: MICROBIOLOGIA / SAUDE AMBIENTAL Año: 2024 Tipo del documento: Article Pais de publicación: Alemania