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Improving hemocompatibility of decellularized vascular tissue by structural modification of collagen fiber.
Mahara, Atsushi; Ota, Satoki; Le, Hue Thi; Shimizu, Kaito; Soni, Raghav; Kojima, Kentaro; Hirano, Yoshiaki; Kakinoki, Sachiro; Yamaoka, Tetsuji.
Afiliación
  • Mahara A; Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, Kishibe-shim Machi, Suita, Osaka 564-8565, Japan. Electronic address: mahara@ncvc.go.jp.
  • Ota S; Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, Kishibe-shim Machi, Suita, Osaka 564-8565, Japan; Faculty of Chemistry, Materials and Bioengineering, Kansai University, 3-3-35 Yamatecho, Suita, Osaka 565-8680, Japan.
  • Le HT; Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, Kishibe-shim Machi, Suita, Osaka 564-8565, Japan.
  • Shimizu K; Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, Kishibe-shim Machi, Suita, Osaka 564-8565, Japan; Faculty of Chemistry, Materials and Bioengineering, Kansai University, 3-3-35 Yamatecho, Suita, Osaka 565-8680, Japan.
  • Soni R; Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, Kishibe-shim Machi, Suita, Osaka 564-8565, Japan.
  • Kojima K; Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, Kishibe-shim Machi, Suita, Osaka 564-8565, Japan; Faculty of Chemistry, Materials and Bioengineering, Kansai University, 3-3-35 Yamatecho, Suita, Osaka 565-8680, Japan.
  • Hirano Y; Faculty of Chemistry, Materials and Bioengineering, Kansai University, 3-3-35 Yamatecho, Suita, Osaka 565-8680, Japan.
  • Kakinoki S; Faculty of Chemistry, Materials and Bioengineering, Kansai University, 3-3-35 Yamatecho, Suita, Osaka 565-8680, Japan.
  • Yamaoka T; Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, Kishibe-shim Machi, Suita, Osaka 564-8565, Japan. Electronic address: yamtet@ncvc.go.jp.
Int J Biol Macromol ; 269(Pt 1): 132040, 2024 Jun.
Article en En | MEDLINE | ID: mdl-38702003
ABSTRACT
Decellularized vascular tissue has high potential as a tissue-engineered vascular graft because of its similarity to native vessels in terms of mechanical strength. However, exposed collagen on the tissue induces blood coagulation, and low hemocompatibility is a major obstacle to its vascular application. Here we report that freeze-drying and ethanol treatment effectively modify collagen fiber structure and drastically reduce blood coagulation on the graft surface without exogenous chemical modification. Decellularized carotid artery of ostrich was treated with freeze-drying and ethanol solution at concentrations ranging between 5 and 99.5 %. Collagen fiber distance in the graft was narrowed by freeze-drying, and the non-helical region increased by ethanol treatment. Although in vitro blood coagulation pattern was similar on the grafts, platelet adhesion on the grafts was largely suppressed by freeze-drying and ethanol treatments. Ex vivo blood circulation tests also indicated that the adsorption of platelets and Von Willebrand Factor was largely reduced to approximately 80 % by ethanol treatment. These results indicate that structural modification of collagen fibers in decellularized tissue reduces blood coagulation on the surface by inhibiting platelet adhesion.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Coagulación Sanguínea / Adhesividad Plaquetaria / Colágeno Límite: Animals / Humans Idioma: En Revista: Int J Biol Macromol Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Coagulación Sanguínea / Adhesividad Plaquetaria / Colágeno Límite: Animals / Humans Idioma: En Revista: Int J Biol Macromol Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos