Your browser doesn't support javascript.
loading
Longitudinal Fluctuations in Protein Concentrations and Higher-Order Structures in the Plasma Proteome of Kidney Failure Patients Subjected to a Kidney Transplant.
Kalaidopoulou Nteak, Sofia; Völlmy, Franziska; Lukassen, Marie V; van den Toorn, Henk; den Boer, Maurits A; Bondt, Albert; van der Lans, Sjors P A; Haas, Pieter-Jan; van Zuilen, Arjan D; Rooijakkers, Suzan H M; Heck, Albert J R.
Afiliación
  • Kalaidopoulou Nteak S; Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, University of Utrecht, Utrecht 3584 CH, The Netherlands.
  • Völlmy F; Netherlands Proteomics Center, Utrecht 3584 CH, The Netherlands.
  • Lukassen MV; Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, University of Utrecht, Utrecht 3584 CH, The Netherlands.
  • van den Toorn H; Netherlands Proteomics Center, Utrecht 3584 CH, The Netherlands.
  • den Boer MA; Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, University of Utrecht, Utrecht 3584 CH, The Netherlands.
  • Bondt A; Netherlands Proteomics Center, Utrecht 3584 CH, The Netherlands.
  • van der Lans SPA; Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, University of Utrecht, Utrecht 3584 CH, The Netherlands.
  • Haas PJ; Netherlands Proteomics Center, Utrecht 3584 CH, The Netherlands.
  • van Zuilen AD; Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, University of Utrecht, Utrecht 3584 CH, The Netherlands.
  • Rooijakkers SHM; Netherlands Proteomics Center, Utrecht 3584 CH, The Netherlands.
  • Heck AJR; Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, University of Utrecht, Utrecht 3584 CH, The Netherlands.
J Proteome Res ; 23(6): 2124-2136, 2024 Jun 07.
Article en En | MEDLINE | ID: mdl-38701233
ABSTRACT
Using proteomics and complexome profiling, we evaluated in a year-long study longitudinal variations in the plasma proteome of kidney failure patients, prior to and after a kidney transplantation. The post-transplant period was complicated by bacterial infections, resulting in dramatic changes in the proteome, attributed to an acute phase response (APR). As positive acute phase proteins (APPs), being elevated upon inflammation, we observed the well-described C-reactive protein and Serum Amyloid A (SAA), but also Fibrinogen, Haptoglobin, Leucine-rich alpha-2-glycoprotein, Lipopolysaccharide-binding protein, Alpha-1-antitrypsin, Alpha-1-antichymotrypsin, S100, and CD14. As negative APPs, being downregulated upon inflammation, we identified the well-documented Serotransferrin and Transthyretin, but added Kallistatin, Heparin cofactor 2, and interalpha-trypsin inhibitor heavy chain H1 and H2 (ITIH1, ITIH2). For the patient with the most severe APR, we performed plasma complexome profiling by SEC-LC-MS on all longitudinal samples. We observed that several plasma proteins displaying alike concentration patterns coelute and form macromolecular complexes. By complexome profiling, we expose how SAA1 and SAA2 become incorporated into high-density lipid particles, replacing largely Apolipoprotein (APO)A1 and APOA4. Overall, our data highlight that the combination of in-depth longitudinal plasma proteome and complexome profiling can shed further light on correlated variations in the abundance of several plasma proteins upon inflammatory events.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Sanguíneas / Trasplante de Riñón / Proteoma Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: J Proteome Res Asunto de la revista: BIOQUIMICA Año: 2024 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Sanguíneas / Trasplante de Riñón / Proteoma Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: J Proteome Res Asunto de la revista: BIOQUIMICA Año: 2024 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Estados Unidos