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Syndecan-4 is required for early-stage repair responses during zebrafish heart regeneration.
Lai, Zih-Yin; Yang, Chung-Chi; Chen, Po-Hsun; Chen, Wei-Chen; Lai, Ting-Yu; Lu, Guan-Yun; Yang, Chiao-Yu; Wang, Ko-Ying; Liu, Wei-Cen; Chen, Yu-Chieh; Liu, Lawrence Yu-Min; Chuang, Yung-Jen.
Afiliación
  • Lai ZY; School of Medicine, National Tsing Hua University, Hsinchu, 300044, Taiwan, ROC.
  • Yang CC; Institute of Bioinformatics and Structural Biology, National Tsing Hua University, Hsinchu, 300044, Taiwan, ROC.
  • Chen PH; School of Medicine, National Tsing Hua University, Hsinchu, 300044, Taiwan, ROC.
  • Chen WC; Institute of Bioinformatics and Structural Biology, National Tsing Hua University, Hsinchu, 300044, Taiwan, ROC.
  • Lai TY; Division of Cardiovascular Medicine, Taoyuan Armed Forces General Hospital, Taoyuan City, 325208, Taiwan, ROC.
  • Lu GY; Cardiovascular Division, Tri-Service General Hospital, National Defense Medical Center, Taipei City, 114201, Taiwan, ROC.
  • Yang CY; School of Medicine, National Tsing Hua University, Hsinchu, 300044, Taiwan, ROC.
  • Wang KY; Institute of Bioinformatics and Structural Biology, National Tsing Hua University, Hsinchu, 300044, Taiwan, ROC.
  • Liu WC; School of Medicine, National Tsing Hua University, Hsinchu, 300044, Taiwan, ROC.
  • Chen YC; Institute of Bioinformatics and Structural Biology, National Tsing Hua University, Hsinchu, 300044, Taiwan, ROC.
  • Liu LY; School of Medicine, National Tsing Hua University, Hsinchu, 300044, Taiwan, ROC.
  • Chuang YJ; Institute of Bioinformatics and Structural Biology, National Tsing Hua University, Hsinchu, 300044, Taiwan, ROC.
Mol Biol Rep ; 51(1): 604, 2024 May 03.
Article en En | MEDLINE | ID: mdl-38700644
ABSTRACT

BACKGROUND:

The healing process after a myocardial infarction (MI) in humans involves complex events that replace damaged tissue with a fibrotic scar. The affected cardiac tissue may lose its function permanently. In contrast, zebrafish display a remarkable capacity for scar-free heart regeneration. Previous studies have revealed that syndecan-4 (SDC4) regulates inflammatory response and fibroblast activity following cardiac injury in higher vertebrates. However, whether and how Sdc4 regulates heart regeneration in highly regenerative zebrafish remains unknown. METHODS AND

RESULTS:

This study showed that sdc4 expression was differentially regulated during zebrafish heart regeneration by transcriptional analysis. Specifically, sdc4 expression increased rapidly and transiently in the early regeneration phase upon ventricular cryoinjury. Moreover, the knockdown of sdc4 led to a significant reduction in extracellular matrix protein deposition, immune cell accumulation, and cell proliferation at the lesion site. The expression of tgfb1a and col1a1a, as well as the protein expression of Fibronectin, were all down-regulated under sdc4 knockdown. In addition, we verified that sdc4 expression was required for cardiac repair in zebrafish via in vivo electrocardiogram analysis. Loss of sdc4 expression caused an apparent pathological Q wave and ST elevation, which are signs of human MI patients.

CONCLUSIONS:

Our findings support that Sdc4 is required to mediate pleiotropic repair responses in the early stage of zebrafish heart regeneration.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regeneración / Pez Cebra / Sindecano-4 / Corazón Límite: Animals Idioma: En Revista: Mol Biol Rep Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regeneración / Pez Cebra / Sindecano-4 / Corazón Límite: Animals Idioma: En Revista: Mol Biol Rep Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos