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Globally occurring pelagiphage infections create ribosome-deprived cells.
Brüwer, Jan D; Sidhu, Chandni; Zhao, Yanlin; Eich, Andreas; Rößler, Leonard; Orellana, Luis H; Fuchs, Bernhard M.
Afiliación
  • Brüwer JD; Max Planck Institute for Marine Microbiology, 28359, Bremen, Germany. jbruewer@mpi-bremen.de.
  • Sidhu C; Max Planck Institute for Marine Microbiology, 28359, Bremen, Germany.
  • Zhao Y; College of Juncao Science and Ecology, Fujian Agriculture and Forestry University, Fuzhou, China.
  • Eich A; PSL Research University: EPHE-UPVD-CNRS,UAR 3278 CRIOBE, Moorea, French Polynesia.
  • Rößler L; Max Planck Institute for Marine Microbiology, 28359, Bremen, Germany.
  • Orellana LH; Max Planck Institute for Marine Microbiology, 28359, Bremen, Germany.
  • Fuchs BM; Max Planck Institute for Marine Microbiology, 28359, Bremen, Germany. bfuchs@mpi-bremen.de.
Nat Commun ; 15(1): 3715, 2024 May 02.
Article en En | MEDLINE | ID: mdl-38698041
ABSTRACT
Phages play an essential role in controlling bacterial populations. Those infecting Pelagibacterales (SAR11), the dominant bacteria in surface oceans, have been studied in silico and by cultivation attempts. However, little is known about the quantity of phage-infected cells in the environment. Using fluorescence in situ hybridization techniques, we here show pelagiphage-infected SAR11 cells across multiple global ecosystems and present evidence for tight community control of pelagiphages on the SAR11 hosts in a case study. Up to 19% of SAR11 cells were phage-infected during a phytoplankton bloom, coinciding with a ~90% reduction in SAR11 cell abundance within 5 days. Frequently, a fraction of the infected SAR11 cells were devoid of detectable ribosomes, which appear to be a yet undescribed possible stage during pelagiphage infection. We dubbed such cells zombies and propose, among other possible explanations, a mechanism in which ribosomal RNA is used as a resource for the synthesis of new phage genomes. On a global scale, we detected phage-infected SAR11 and zombie cells in the Atlantic, Pacific, and Southern Oceans. Our findings illuminate the important impact of pelagiphages on SAR11 populations and unveil the presence of ribosome-deprived zombie cells as part of the infection cycle.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ribosomas / Bacteriófagos Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2024 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ribosomas / Bacteriófagos Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2024 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido