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Dysregulation of phosphoenolpyruvate carboxykinase in cancers: A comprehensive analysis.
Xue, Shuaishuai; Cai, Yonghua; Liu, Jun; Ji, Ke; Yi, Peiyao; Long, Hao; Zhang, Xian; Li, Peng; Song, Ye.
Afiliación
  • Xue S; Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
  • Cai Y; Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
  • Liu J; Department of Neurosurgery, The 2(nd) affiliated hospital Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi 330000, China.
  • Ji K; Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
  • Yi P; Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
  • Long H; Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
  • Zhang X; Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. Electronic address: zxa@smu.edu.cn.
  • Li P; Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. Electronic address: zhimeng_li@163.com.
  • Song Y; Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. Electronic address: songye@smu.edu.cn.
Cell Signal ; 120: 111198, 2024 Aug.
Article en En | MEDLINE | ID: mdl-38697449
ABSTRACT

BACKGROUND:

Phosphoenolpyruvate carboxykinase (PEPCK) plays a crucial role in gluconeogenesis, glycolysis, and the tricarboxylic acid cycle by converting oxaloacetate into phosphoenolpyruvate. Two distinct isoforms of PEPCK, specifically cytosolic PCK1 and mitochondrial PCK2, have been identified. Nevertheless, the comprehensive understanding of their dysregulation in pan-cancer and their potential mechanism contributing to signaling transduction pathways remains elusive.

METHODS:

We conducted comprehensive analyses of PEPCK gene expression across 33 diverse cancer types using data from The Cancer Genome Atlas (TCGA). Multiple public databases such as HPA, TIMER 2.0, GEPIA2, cBioPortal, UALCAN, CancerSEA, and String were used to investigate protein levels, prognostic significance, clinical associations, genetic mutations, immune cell infiltration, single-cell sequencing, and functional enrichment analysis in patients with pan-cancer. PEPCK expression was analyzed about different clinical and genetic factors of patients using data from TCGA, GEO, and CGGA databases. Furthermore, the role of PCK2 in Glioma was examined using both in vitro and in vivo experiments.

RESULTS:

The analysis we conducted revealed that the expression of PEPCK is involved in both clinical outcomes and immune cell infiltration. Initially, we verified the high expression of PCK2 in GBM cells and its role in metabolic reprogramming and proliferation in GBM.

CONCLUSION:

Our study showed a correlation between PEPCK (PCK1 and PCK2) expression with clinical prognosis, gene mutation, and immune infiltrates. These findings identified two possible predictive biomarkers across different cancer types, as well as a comprehensive analysis of PCK2 expression in various tumors, with a focus on GBM.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfoenolpiruvato Carboxiquinasa (GTP) / Neoplasias Límite: Animals / Humans Idioma: En Revista: Cell Signal Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfoenolpiruvato Carboxiquinasa (GTP) / Neoplasias Límite: Animals / Humans Idioma: En Revista: Cell Signal Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido