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Assessment of fish protein hydrolysate as a substitute for fish meal in white shrimp diets: Impact on growth, immune response, and resistance against Vibrio parahaemolyticus infection.
Tran, Hoang Trieu Quan; Ho, Thi Hang; Nan, Fan-Hua; Liu, Chun-Hung; Hu, Yeh-Fang; Chong, Chou Min; de Cruz, Clement R; Karim, Murni; Liu, Ting-Jui; Kuo, I-Pei; Lee, Po-Tsang.
Afiliación
  • Tran HTQ; Department of Aquaculture, National Taiwan Ocean University, Keelung City, Taiwan.
  • Ho TH; Department of Aquaculture, National Taiwan Ocean University, Keelung City, Taiwan.
  • Nan FH; Department of Aquaculture, National Taiwan Ocean University, Keelung City, Taiwan.
  • Liu CH; Department of Aquaculture, National Pingtung University of Science and Technology, Pingtung, Taiwan.
  • Hu YF; Department of Aquaculture, National Taiwan Ocean University, Keelung City, Taiwan.
  • Chong CM; International Institute of Aquaculture and Aquatic Sciences (I-AQUAS), Universiti Putra Malaysia, Port Dickson, Negeri Sembilan, Malaysia.
  • de Cruz CR; International Institute of Aquaculture and Aquatic Sciences (I-AQUAS), Universiti Putra Malaysia, Port Dickson, Negeri Sembilan, Malaysia.
  • Karim M; International Institute of Aquaculture and Aquatic Sciences (I-AQUAS), Universiti Putra Malaysia, Port Dickson, Negeri Sembilan, Malaysia.
  • Liu TJ; Department of Aquaculture, National Taiwan Ocean University, Keelung City, Taiwan.
  • Kuo IP; Department of Aquaculture, National Taiwan Ocean University, Keelung City, Taiwan; Freshwater Aquaculture Research Center Chupei Station, Fisheries Research Institute, Ministry of Agriculture, Hsinchu, Taiwan. Electronic address: ipkuo@mail.tfrin.gov.tw.
  • Lee PT; Department of Aquaculture, National Taiwan Ocean University, Keelung City, Taiwan. Electronic address: leepotsang@email.ntou.edu.tw.
Fish Shellfish Immunol ; 150: 109597, 2024 Jul.
Article en En | MEDLINE | ID: mdl-38697373
ABSTRACT
This study investigated the effects of fish protein hydrolysate derived from barramundi on growth performance, muscle composition, immune response, disease resistance, histology and gene expression in white shrimp (Penaeus vannamei). In vitro studies demonstrated FPH enhanced mRNA expressions of key immune-related genes and stimulated reactive oxygen species (ROS) production and phagocytic activity in shrimp hemocytes. To evaluate the effects of substituting fish meal with FPH in vivo, four isoproteic (43 %), isolipidic (6 %), and isoenergetic diets (489 kcal/100 g) were formulated with fish meal substitution levels of 0 % (control), 30 % (FPH30), 65 % (FPH65), and 100 % (FPH100). After 8-week feeding, the growth performance of FPH65 and FPH100 were significantly lower than that of control and FPH30 (p < 0.05). Similarly, the midgut histological examination revealed the wall thickness and villi height of FPH100 were significantly lower than those of control (p < 0.05). The shrimps were received the challenge of AHPND + Vibrio parahaemolyticus at week 4 and 8. All FPH-fed groups significantly enhanced resistance against Vibrio parahaemolyticus at week 4 (p < 0.05). However, this protective effect diminished after long-period feeding. No significant difference of survival rate was observed among all groups at week 8 (p > 0.05). The expressions of immune-related genes were analyzed at week 4 before and after challenge. In control group, V. parahaemolyticus significantly elevated SOD in hepatopancreas and Muc 19, trypsin, Midline-fas, and GPx in foregut (p < 0.05). Moreover, hepatopancreatic SOD of FPH65 and FPH100 were significantly higher than that of control before challenge (p < 0.05). Immune parameters were measured at week 8. Compared with control, the phagocytic index of FPH 30 was significantly higher (p < 0.05). However, dietary FPH did not alter ROS production, phenoloxidase activity, phagocytic rate, and total hemocyte count (p > 0.05). These findings suggest that FPH30 holds promise as a feed without adverse impacts on growth performance while enhancing the immunological response of white shrimp.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hidrolisados de Proteína / Vibrio parahaemolyticus / Penaeidae / Dieta / Inmunidad Innata / Alimentación Animal Límite: Animals Idioma: En Revista: Fish Shellfish Immunol Asunto de la revista: BIOLOGIA / MEDICINA VETERINARIA Año: 2024 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hidrolisados de Proteína / Vibrio parahaemolyticus / Penaeidae / Dieta / Inmunidad Innata / Alimentación Animal Límite: Animals Idioma: En Revista: Fish Shellfish Immunol Asunto de la revista: BIOLOGIA / MEDICINA VETERINARIA Año: 2024 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Reino Unido