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Antibacterial Activity of Epigallocatechin-3-gallate (EGCG) Loaded Lipid-chitosan Hybrid Nanoparticle against Planktonic Microorganisms.
Moreno, Ana Paula Dias; Marcato, Priscyla Daniely; Silva, Letícia Bueno; Salvador, Sérgio Luiz de Souza; Arco, Marina Constante Gabriel Del; Moraes, Juliana Cristina Biazzoto de; Silva, Roberto Santana da; Rossi, Andiara De.
Afiliación
  • Moreno APD; Department of Pediatric Dentistry - School of Dentistry of Ribeirão Preto, University of São Paulo.
  • Marcato PD; Department of Pharmaceutical Science, GNanobio Lab - School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo.
  • Silva LB; Department of Pharmaceutical Science, GNanobio Lab - School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo.
  • Salvador SLS; Department of Clinical Analyses, - School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo.
  • Arco MCGD; Department of Clinical Analyses, - School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo.
  • Moraes JCB; Department of Analytical chemistry - School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo.
  • Silva RSD; Department of Analytical chemistry - School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo.
  • Rossi A; Department of Pediatric Dentistry - School of Dentistry of Ribeirão Preto, University of São Paulo.
J Oleo Sci ; 73(5): 709-716, 2024.
Article en En | MEDLINE | ID: mdl-38692893
ABSTRACT
Epigallocatechin-3-gallate (EGCG), a polyphenol derived from Green Tea, is one of the sources of natural bioactive compounds which are currently being developed as medicinal ingredients. Besides other biological activities, this natural compound exhibits anti-cariogenic effects. However, EGCG has low physical-chemical stability and poor bioavailability. Thus, the purpose of this study was to develop and characterize lipid-chitosan hybrid nanoparticle with EGCG and to evaluate its in vitro activity against cariogenic planktonic microorganisms. Lipid-chitosan hybrid nanoparticle (LCHNP-EGCG) were prepared by emulsion and sonication method in one step and characterized according to diameter, polydispersity index (PdI), zeta potential (ZP), encapsulation efficiency (EE), mucoadhesion capacity and morphology. Strains of Streptococcus mutans, Streptococcus sobrinus and Lactobacillus casei were treated with LCHNP- EGCG, and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were evaluated. LCHNP-EGCG exhibited a size of 217.3 ± 5.1 nm with a low polydispersity index (0.17) and positive zeta potential indicating the presence of chitosan on the lipid nanoparticle surface (+33.7 mV). The LCHNP-EGCG showed a spherical morphology, high stability and a mucoadhesive property due to the presence of chitosan coating. In addition, the EGCG encapsulation efficiency was 96%. A reduction of almost 15-fold in the MIC and MBC against the strains was observed when EGCG was encapsulated in LCHNP, indicating the potential of EGCG encapsulation in lipid-polymer hybrid nanoparticles. Taking the results together, the LCHNP-EGCG could be an interesting system to use in dental care due to their nanometric size, mucoadhesive properties high antibacterial activity against relevant planktonic microorganisms.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Streptococcus mutans / Pruebas de Sensibilidad Microbiana / Catequina / Quitosano / Nanopartículas / Antibacterianos Idioma: En Revista: J Oleo Sci Asunto de la revista: BIOQUIMICA Año: 2024 Tipo del documento: Article Pais de publicación: Japón
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Streptococcus mutans / Pruebas de Sensibilidad Microbiana / Catequina / Quitosano / Nanopartículas / Antibacterianos Idioma: En Revista: J Oleo Sci Asunto de la revista: BIOQUIMICA Año: 2024 Tipo del documento: Article Pais de publicación: Japón