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Dual-mode fluorescence and colorimetric smartphone-based sensing platform with oxidation-induced self-assembled nanoflowers for sarcosine detection.
Liu, Peng; Sun, Qian; Gai, Zhexu; Yang, Fei; Yang, Yanzhao.
Afiliación
  • Liu P; Key Laboratory for Special Functional Aggregate Materials of Education Ministry, School of Chemistry and Chemical Engineering, Shandong University, Jinan, Shandong, 250100, China.
  • Sun Q; Key Laboratory for Special Functional Aggregate Materials of Education Ministry, School of Chemistry and Chemical Engineering, Shandong University, Jinan, Shandong, 250100, China.
  • Gai Z; Key Laboratory for Special Functional Aggregate Materials of Education Ministry, School of Chemistry and Chemical Engineering, Shandong University, Jinan, Shandong, 250100, China.
  • Yang F; School of Pharmaceutical Sciences, Shandong University, Jinan, 250012, Shandong, China. Electronic address: yangfei@sdu.edu.cn.
  • Yang Y; Key Laboratory for Special Functional Aggregate Materials of Education Ministry, School of Chemistry and Chemical Engineering, Shandong University, Jinan, Shandong, 250100, China. Electronic address: yzhayang@sdu.edu.cn.
Anal Chim Acta ; 1306: 342586, 2024 Jun 01.
Article en En | MEDLINE | ID: mdl-38692787
ABSTRACT

BACKGROUND:

Early prostatic cancer (PCa) diagnosis significantly improves the chances of successful treatment and enhances patient survival rates. Traditional enzyme cascade-based early cancer detection methods offer efficiency and signal amplification but are limited by cost, complexity, and enzyme dependency, affecting stability and practicality. Meanwhile, sarcosine (Sar) is commonly considered a biomarker for PCa development. It is essential to develop a Sar detection method based on cascade reactions, which should be efficient, low skill requirement, and suitable for on-site testing.

RESULTS:

To address this, our study introduces the synthesis of organic-inorganic self-assembled nanoflowers to optimize existing detection methods. The Sar oxidase (SOX)-inorganic hybrid nanoflowers (Cu3(PO4)2Ce@SOX) possess inherent fluorescent properties and excellent peroxidase activity, coupled with efficient enzyme loading. Based on this, we have developed a dual-mode multi-enzyme cascade nanoplatform combining fluorescence and colorimetric methods for the detection of Sar. The encapsulation yield of Cu3(PO4)2Ce@SOX reaches 84.5 %, exhibiting a remarkable enhancement in catalytic activity by 1.26-1.29 fold compared to free SOX. The present study employing a dual-signal mechanism encompasses 'turn-off' fluorescence signals ranging from 0.5 µM to 60 µM, with a detection limit of 0.226 µM, and 'turn-on' colorimetric signals ranging from 0.18 µM to 60 µM, with a detection limit of 0.120 µM.

SIGNIFICANCE:

Furthermore, our study developed an intelligent smartphone sensor system utilizing cotton swabs for real-time analysis of Sar without additional instruments. The nano-platform exhibits exceptional repeatability and stability, rendering it well-suited for detecting Sar in authentic human urine samples. This innovation allows for immediate analysis, offering valuable insights for portable and efficient biosensors applicable to Sar and other analytes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oxidación-Reducción / Sarcosina / Colorimetría / Teléfono Inteligente Límite: Humans Idioma: En Revista: Anal Chim Acta Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oxidación-Reducción / Sarcosina / Colorimetría / Teléfono Inteligente Límite: Humans Idioma: En Revista: Anal Chim Acta Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos