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Lats1 and Lats2 regulate YAP and TAZ activity to control the development of mouse Sertoli cells.
Abou Nader, Nour; Charrier, Laureline; Meisnsohn, Marie-Charlotte; Banville, Laurence; Deffrennes, Bérengère; St-Jean, Guillaume; Boerboom, Derek; Zamberlam, Gustavo; Brind'Amour, Julie; Pépin, David; Boyer, Alexandre.
Afiliación
  • Abou Nader N; Centre de Recherche en Reproduction et Fertilité, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Quebec, Canada.
  • Charrier L; Centre de Recherche en Reproduction et Fertilité, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Quebec, Canada.
  • Meisnsohn MC; Pediatric Surgical Research Laboratories, Massachusetts General Hospital, Boston, Massachusetts, USA.
  • Banville L; Centre de Recherche en Reproduction et Fertilité, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Quebec, Canada.
  • Deffrennes B; Centre de Recherche en Reproduction et Fertilité, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Quebec, Canada.
  • St-Jean G; École Nationale Vétérinaire d'Alfort, Maisons-Alfort, France.
  • Boerboom D; Centre de Recherche en Reproduction et Fertilité, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Quebec, Canada.
  • Zamberlam G; Centre de Recherche en Reproduction et Fertilité, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Quebec, Canada.
  • Brind'Amour J; Centre de Recherche en Reproduction et Fertilité, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Quebec, Canada.
  • Pépin D; Centre de Recherche en Reproduction et Fertilité, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Quebec, Canada.
  • Boyer A; Pediatric Surgical Research Laboratories, Massachusetts General Hospital, Boston, Massachusetts, USA.
FASEB J ; 38(9): e23633, 2024 May 15.
Article en En | MEDLINE | ID: mdl-38690712
ABSTRACT
Recent reports suggest that the Hippo signaling pathway regulates testis development, though its exact roles in Sertoli cell differentiation remain unknown. Here, we examined the functions of the main Hippo pathway kinases, large tumor suppressor homolog kinases 1 and 2 (Lats1 and Lats2) in developing mouse Sertoli cells. Conditional inactivation of Lats1/2 in Sertoli cells resulted in the disorganization and overgrowth of the testis cords, the induction of a testicular inflammatory response and germ cell apoptosis. Stimulated by retinoic acid 8 (STRA8) expression in germ cells additionally suggested that germ cells may have been preparing to enter meiosis prior to their loss. Gene expression analyses of the developing testes of conditional knockout animals further suggested impaired Sertoli cell differentiation, epithelial-to-mesenchymal transition, and the induction of a specific set of genes associated with Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ)-mediated integrin signaling. Finally, the involvement of YAP/TAZ in Sertoli cell differentiation was confirmed by concomitantly inactivating Yap/Taz in Lats1/2 conditional knockout model, which resulted in a partial rescue of the testicular phenotypic changes. Taken together, these results identify Hippo signaling as a crucial pathway for Sertoli cell development and provide novel insight into Sertoli cell fate maintenance.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células de Sertoli / Diferenciación Celular / Proteínas Serina-Treonina Quinasas / Proteínas Supresoras de Tumor / Proteínas Adaptadoras Transductoras de Señales / Proteínas Señalizadoras YAP Límite: Animals Idioma: En Revista: FASEB J Asunto de la revista: BIOLOGIA / FISIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células de Sertoli / Diferenciación Celular / Proteínas Serina-Treonina Quinasas / Proteínas Supresoras de Tumor / Proteínas Adaptadoras Transductoras de Señales / Proteínas Señalizadoras YAP Límite: Animals Idioma: En Revista: FASEB J Asunto de la revista: BIOLOGIA / FISIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos