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mmu-miR-185 regulates osteoclasts differentiation and migration by targeting Btk.
He, Dan; Jiao, Yueying; Xu, Jian; Luo, Junjie; Cui, Yaqi; Han, Xiabing; Zhao, Hongshan.
Afiliación
  • He D; Department of Medical Genetics, Peking University School of Basic Medical Sciences, Beijing, China.
  • Jiao Y; Department of Medical Genetics, Peking University School of Basic Medical Sciences, Beijing, China.
  • Xu J; Department of Anatomy, Histology and Embryology, Peking University School of Basic Medical Sciences, Beijing, China.
  • Luo J; Department of Medical Genetics, Peking University School of Basic Medical Sciences, Beijing, China.
  • Cui Y; Department of Medical Genetics, Peking University School of Basic Medical Sciences, Beijing, China.
  • Han X; Department of Medical Genetics, Peking University School of Basic Medical Sciences, Beijing, China.
  • Zhao H; Department of Medical Genetics, Peking University School of Basic Medical Sciences, Beijing, China.
J Gene Med ; 26(5): e3687, 2024 May.
Article en En | MEDLINE | ID: mdl-38690623
ABSTRACT

BACKGROUND:

Bones undergo a constant remodeling, a process involving osteoclast-mediated bone resorption and osteoblast-mediated bone formation, crucial for maintaining healthy bone mass. We previously observed that miR-185 depletion may promote bone formation by regulating Bgn expression and the BMP/Smad signaling pathway. However, the effects of miR-185-5p on the osteoclasts and bone remodeling have not been elucidated, warranting further exploration.

METHODS:

Tartrate-resistant acid phosphatase staining was utilized to assess the differentiation ability of bone marrow mononuclear macrophages (BMMs) from mmu-miR-185 gene knockout (KO) mice and wild-type (WT) mice. A reverse transcriptase-quantitative PCR was conducted to compare differences in miR-185-5p and osteoclast marker molecules, including Trap, Dcstamp, Ctsk and Nfatc1, between the KO group and WT group BMMs. Western blot analysis was employed to observe the expression of osteoclast marker molecules. A cell-counting kit-8 was used to analyze cell proliferation ability. Transwell experiments were conducted to detect cell migration. Dual-luciferase reporter assays were employed to confirm whether Btk is a downstream target gene of miR-185-5p.

RESULTS:

miR-185 depletion promoted osteoclast differentiation in bone marrow-derived monocytes/macrophages. Overexpression of miR-185-5p in RAW264.7 cells inhibited differentiation and migration of osteoclasts. Furthermore, Btk was identified as a downstream target gene of miR-185-5p, suggesting that miR-185-5p may inhibit osteoclast differentiation and migration by targeting Btk.

CONCLUSIONS:

miR-185 regulates osteoclasts differentiation, with overexpression of miR-185-5p inhibiting osteoclast differentiation and migration in vitro. Additionally, miR-185-5p may modulate osteoclastic differentiation and migration by regulating Btk expression.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteoclastos / Diferenciación Celular / Movimiento Celular / Ratones Noqueados / MicroARNs / Agammaglobulinemia Tirosina Quinasa Límite: Animals Idioma: En Revista: J Gene Med Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA MEDICA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteoclastos / Diferenciación Celular / Movimiento Celular / Ratones Noqueados / MicroARNs / Agammaglobulinemia Tirosina Quinasa Límite: Animals Idioma: En Revista: J Gene Med Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA MEDICA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido