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Synthesis, Characterization, and Antifungal Activity of Benzimidazole-Grafted Chitosan against Aspergillus flavus.
Liu, Jing; Chen, Hao; Lv, Yan; Wu, Huixiang; Yang, Li-Jun; Zhang, Jiahui; Huang, Jianying; Wang, Wenjie.
Afiliación
  • Liu J; College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, Zhejiang 310018, PR. China.
  • Chen H; College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, Zhejiang 310018, PR. China.
  • Lv Y; College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, Zhejiang 310018, PR. China.
  • Wu H; College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, Zhejiang 310018, PR. China.
  • Yang LJ; College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, Zhejiang 310018, PR. China.
  • Zhang J; Hangzhou Sanyan Biotechnology Co., Ltd., Hangzhou, Zhejiang 310018, PR. China.
  • Huang J; Xiuzheng Bio-Medicine Research Institute Co., Ltd., Hangzhou, Zhejiang 310018, PR. China.
  • Wang W; College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, Zhejiang 310018, PR. China.
J Agric Food Chem ; 72(19): 11185-11194, 2024 May 15.
Article en En | MEDLINE | ID: mdl-38687832
ABSTRACT
Aspergillus flavus contamination in agriculture and food industries poses threats to human health, leading to a requirement of a safe and effective method to control fungal contamination. Chitosan-based nitrogen-containing derivatives have attracted much attention due to their safety and enhanced antimicrobial applications. Herein, a new benzimidazole-grafted chitosan (BAC) was synthesized by linking the chitosan (CS) with a simple benzimidazole compound, 2-benzimidazolepropionic acid (BA). The characterization of BAC was confirmed by Fourier transform infrared (FTIR) spectroscopy and nuclear magnetic resonance spectroscopy (1H and 13C NMR). Then, the efficiency of BAC against A. flavus ACCC 32656 was investigated in terms of spore germination, mycelial growth, and aflatoxin production. BAC showed a much better antifungal effect than CS and BA. The minimum inhibitory concentration (MIC) value was 1.25 mg/mL for BAC, while the highest solubility of CS (16.0 mg/mL) or BA (4.0 mg/mL) could not completely inhibit the growth of A. flavus. Furthermore, results showed that BAC inhibited spore germination and elongation by affecting ergosterol biosynthesis and the cell membrane integrity, leading to the permeabilization of the plasma membrane and leakage of intracellular content. The production of aflatoxin was also inhibited when treated with BAC. These findings indicate that benzimidazole-derived natural CS has the potential to be used as an ideal antifungal agent for food preservation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aspergillus flavus / Bencimidazoles / Pruebas de Sensibilidad Microbiana / Quitosano / Fungicidas Industriales Idioma: En Revista: J Agric Food Chem Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aspergillus flavus / Bencimidazoles / Pruebas de Sensibilidad Microbiana / Quitosano / Fungicidas Industriales Idioma: En Revista: J Agric Food Chem Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos