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A sonication-filtration method for acquisition of integrated bacterial communities from phycosphere.
Cai, Chao; Chen, Yuerong; Ohore, Okugbe Ebiotubo; Cai, Guanjing; Cai, Runlin; Wang, Hui.
Afiliación
  • Cai C; Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou, 515063, China; Biology Department and Institute of Marine Sciences, College of Science, Shantou University, Shantou, 515063, China.
  • Chen Y; Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou, 515063, China; Biology Department and Institute of Marine Sciences, College of Science, Shantou University, Shantou, 515063, China.
  • Ohore OE; Key Laboratory of Tropical Diseases Control, National Health Commission, Key Laboratory of Tropical Translational Medicine of Ministry of Education, School of Tropical Medicine, Hainan Medical University, Haikou, Hainan, 571199, China.
  • Cai G; Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou, 515063, China; Biology Department and Institute of Marine Sciences, College of Science, Shantou University, Shantou, 515063, China.
  • Cai R; Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou, 515063, China; Biology Department and Institute of Marine Sciences, College of Science, Shantou University, Shantou, 515063, China.
  • Wang H; Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou, 515063, China; Biology Department and Institute of Marine Sciences, College of Science, Shantou University, Shantou, 515063, China. Electronic address: wanghui@stu.edu.cn.
Mar Environ Res ; 198: 106522, 2024 Jun.
Article en En | MEDLINE | ID: mdl-38685152
ABSTRACT
Bacteria in phycosphere engage in intricate interactions with microalgae by exchanging organic and inorganic matter. However, elucidating the primary roles of bacteria in phycosphere has been a big challenge, due to the lack of adequate methods for separating tightly associated bacteria from microalgal cells. In this study we evaluated several isolation methods including centrifugation, filtration, sonication combined with filtration, and tween lysis followed by sonication and filtration, aiming to efficiently acquire complete bacterial communities from phycosphere. The results demonstrated that the sonication-filtration approach maximally preserves the original characteristics of the bacterial communities. This method will facilitate the acquisition and further analysis of future experimental data.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sonicación / Bacterias / Filtración Idioma: En Revista: Mar Environ Res Asunto de la revista: BIOLOGIA / SAUDE AMBIENTAL / TOXICOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sonicación / Bacterias / Filtración Idioma: En Revista: Mar Environ Res Asunto de la revista: BIOLOGIA / SAUDE AMBIENTAL / TOXICOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido