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Protective anti-chlamydial vaccine regimen-induced CD4+ T cell response mediates early inhibition of pathogenic CD8+ T cell response following genital challenge.
Murthy, Ashlesh K; Wright-McAfee, Erika; Warda, Katerina; Moy, Lindsay N; Bui, Nhi; Musunuri, Tarakarama; Manam, Srikanth; Chako, Clemence Z; Ramsey, Kyle H; Li, Weidang.
Afiliación
  • Murthy AK; College of Veterinary Medicine, Midwestern University, Glendale, AZ 85308, USA.
  • Wright-McAfee E; College of Veterinary Medicine, Midwestern University, Glendale, AZ 85308, USA.
  • Warda K; Arizona College of Osteopathic Medicine, Midwestern University, Glendale, AZ 85308, USA.
  • Moy LN; Arizona College of Osteopathic Medicine, Midwestern University, Glendale, AZ 85308, USA.
  • Bui N; Arizona College of Osteopathic Medicine, Midwestern University, Glendale, AZ 85308, USA.
  • Musunuri T; Arizona College of Osteopathic Medicine, Midwestern University, Glendale, AZ 85308, USA.
  • Manam S; College of Veterinary Medicine, Midwestern University, Glendale, AZ 85308, USA.
  • Chako CZ; College of Veterinary Medicine, Midwestern University, Glendale, AZ 85308, USA.
  • Ramsey KH; College of Veterinary Medicine, Midwestern University, Glendale, AZ 85308, USA.
  • Li W; College of Graduate Studies, Midwestern University, Glendale, AZ 85308, USA.
Pathog Dis ; 822024 Feb 07.
Article en En | MEDLINE | ID: mdl-38684476
ABSTRACT
We have demonstrated previously that TNF-α-producing CD8+ T cells mediate chlamydial pathogenesis, likely in an antigen (Ag)-specific fashion. Here we hypothesize that inhibition of Ag-specific CD8+ T cell response after immunization and/or challenge would correlate with protection against oviduct pathology induced by a protective vaccine regimen. Intranasal (i.n.) live chlamydial elementary body (EB), intramuscular (i.m.) live EB, or i.n. irrelevant antigen, bovine serum albumin (BSA), immunized animals induced near-total protection, 50% protection, or no protection, respectively against oviduct pathology following i.vag. C. muridarum challenge. In these models, we evaluated Ag-specific CD8+ T cell cytokine response at various time-periods after immunization or challenge. The results show protective efficacy of vaccine regimens correlated with reduction of Ag-specific CD8+ T cell TNF-α responses following i.vag. chlamydial challenge, not after immunization. Depletion of CD4+ T cells abrogated, whereas adoptive transfer of Ag-specific CD4+ T cells induced the significant reduction of Ag-specific CD8+ T cell TNF-α response after chlamydial challenge. In conclusion, protective anti-chlamydial vaccine regimens induce Ag-specific CD4+ T cell response that mediate early inhibition of pathogenic CD8+ T cell response following challenge and may serve as a predictive biomarker of protection against Chlamydia -induced chronic pathologies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vacunas Bacterianas / Linfocitos T CD4-Positivos / Infecciones por Chlamydia / Linfocitos T CD8-positivos Límite: Animals Idioma: En Revista: Pathog Dis Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vacunas Bacterianas / Linfocitos T CD4-Positivos / Infecciones por Chlamydia / Linfocitos T CD8-positivos Límite: Animals Idioma: En Revista: Pathog Dis Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos