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Molecular detection and characterization of prevailing Theileria equi genotype in equine from northern India.
Maharana, Biswa Ranjan; Ganguly, Anita; Potliya, Sandeep; Kumar, Binod; Singh, Harpreet; Dash, Aishwarya; Khanna, Sujoy.
Afiliación
  • Maharana BR; Haryana Pashu Vigyan Kendra, Lala Lajpat Rai University of Veterinary and Animal Sciences, Uchani, Karnal 132001, Haryana, India. Electronic address: drbiswaranjanmaharana@gmail.com.
  • Ganguly A; Haryana Pashu Vigyan Kendra, Lala Lajpat Rai University of Veterinary and Animal Sciences, Uchani, Karnal 132001, Haryana, India.
  • Potliya S; Haryana Pashu Vigyan Kendra, Lala Lajpat Rai University of Veterinary and Animal Sciences, Uchani, Karnal 132001, Haryana, India.
  • Kumar B; College of Veterinary and Animal Sciences, BASU, Kishanganj 855107, Bihar, India.
  • Singh H; Haryana Pashu Vigyan Kendra, Lala Lajpat Rai University of Veterinary and Animal Sciences, Uchani, Karnal 132001, Haryana, India.
  • Dash A; ICAR- National Dairy Research Institute, Karnal 132001, Haryana, India.
  • Khanna S; Haryana Pashu Vigyan Kendra, Lala Lajpat Rai University of Veterinary and Animal Sciences, Uchani, Karnal 132001, Haryana, India.
Res Vet Sci ; 173: 105277, 2024 Jun.
Article en En | MEDLINE | ID: mdl-38678846
ABSTRACT
Equine piroplasmosis caused by Theileria equi is a febrile, tick-borne disease of equids. However, there is limited literature about the genotyping of T. equi in India. Blood samples were collected from 202 horses and subjected to microscopy and PCR to detect T. equi. Initially, a universal screening primer pair targeting 18S ribosomal RNA genes common for Babesia caballi and T. equi was employed to amplify the DNA of both parasites. Thereafter additional primers were employed for species-specific detection resulting in amplification of approximately 435 bp specific for T. equi. T.equi was detected in 9.9% and 20.79% of horses screened by microscopy and PCR, respectively. The representative samples confirmed positive by PCR were sequenced, submitted to NCBI (OR651254, OR687254, OR685656, OR650830, OR650834), and used for genotype characterization and phylogenetic analysis. Employing Genetool and MEGA X software, the T. equi Indian isolates and across the globe were compared, and the results demonstrated 99.05-100% and 95.86-100% homologies, respectively. All the T. equi Indian isolates belonged to genotype A. Phylogeny based on the EMA-1 gene of five isolates (OR731831, OR731833, OR731829, OR731830, OR731832) were also characterized by sequencing and support the previous findings. Genotypes C and D, as well as genotypes B and E, exhibited lower levels of evolutionary divergence compared to other genotypes. The EMA-1 gene exhibited limited diversity and might not be the most suitable target for assessing variability within T. equi populations. The findings also reveal a significant association (p < 0.01) between T. equi infection and the presence of ticks.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filogenia / Theileriosis / Theileria / Genotipo / Enfermedades de los Caballos Límite: Animals País/Región como asunto: Asia Idioma: En Revista: Res Vet Sci Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filogenia / Theileriosis / Theileria / Genotipo / Enfermedades de los Caballos Límite: Animals País/Región como asunto: Asia Idioma: En Revista: Res Vet Sci Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido