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Lung fibroblast-derived extracellular vesicles and soluble factors alleviate elastase-induced lung injury.
van der Koog, Luke; Boerrigter, Myrthe J; Gorter, Iris C; Gosens, Reinoud; Nagelkerke, Anika.
Afiliación
  • van der Koog L; Department of Molecular Pharmacology, Groningen Research Institute of Pharmacy, Faculty of Science and Engineering, University of Groningen, Groningen, the Netherlands; GRIAC, Groningen Research Institute for Asthma and COPD, University Medical Center Groningen, Groningen, the Netherlands.
  • Boerrigter MJ; Department of Molecular Pharmacology, Groningen Research Institute of Pharmacy, Faculty of Science and Engineering, University of Groningen, Groningen, the Netherlands; GRIAC, Groningen Research Institute for Asthma and COPD, University Medical Center Groningen, Groningen, the Netherlands.
  • Gorter IC; Aquilo BV, Groningen, the Netherlands.
  • Gosens R; Department of Molecular Pharmacology, Groningen Research Institute of Pharmacy, Faculty of Science and Engineering, University of Groningen, Groningen, the Netherlands; GRIAC, Groningen Research Institute for Asthma and COPD, University Medical Center Groningen, Groningen, the Netherlands.
  • Nagelkerke A; Department of Pharmaceutical Analysis, Groningen Research Institute of Pharmacy, Faculty of Science and Engineering, University of Groningen, Groningen, the Netherlands. Electronic address: a.p.nagelkerke@rug.nl.
Eur J Pharmacol ; 974: 176612, 2024 Jul 05.
Article en En | MEDLINE | ID: mdl-38677537
ABSTRACT
One of the main pathological features of chronic obstructive pulmonary disease (COPD) is the loss of functional alveolar tissue as a consequence of impaired regenerative capacities (emphysema). Recent research suggests that the secretome from mesenchymal cells, particularly extracellular vesicles (EVs), may possess regenerative properties beneficial for lung repair. However, the regenerative potential of the soluble factors (SFs) within the secretome remains largely unexplored in COPD. To this extent, we purified EVs and SFs secreted by lung fibroblasts to generate EV-enriched and SF-enriched fractions, and evaluated their effects on elastase-induced lung injury in both precision-cut lung slices (PCLS) and a mouse model. EV- and SF-enriched fractions were concentrated and purified from the conditioned medium of cultured MRC-5 lung fibroblasts using a combination of ultrafiltration and size exclusion chromatography, and were subsequently characterized according to the MISEV guidelines. Treatment with EV- or SF-enriched concentrates prevented and improved elastase-induced emphysema in PCLS, leading to reduced lung injury and upregulated markers of alveolar epithelial cells (aquaporin 5 and surfactant protein C), indicating potential parenchymal regeneration. Accordingly, prophylactic intratracheal treatment with lung fibroblast-derived EV- and SF-enriched concentrates in vivo attenuated elastase-induced lung tissue destruction, improved lung function, and enhanced gene expression of alveolar epithelial cell markers. Here, alveolar repair not only serves the purpose of facilitating gas exchange, but also by reinstating the essential parenchymal tethering required for optimal airway mechanics. In conclusion, this study highlights the therapeutic potential of both lung fibroblast-derived EV- and SF-enriched concentrates for the treatment of lung injury and emphysema.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Elastasa Pancreática / Fibroblastos / Vesículas Extracelulares / Pulmón Límite: Animals / Humans / Male Idioma: En Revista: Eur J Pharmacol Año: 2024 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Elastasa Pancreática / Fibroblastos / Vesículas Extracelulares / Pulmón Límite: Animals / Humans / Male Idioma: En Revista: Eur J Pharmacol Año: 2024 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Países Bajos