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Rapid and sensitive parallel on-site detection of antibiotics and resistance genes in aquatic environments using evanescent wave dual-color fluorescence fiber-embedded optofluidic nanochip.
Chen, Dan; Xu, Wenjuan; Lu, Yongkai; Zhuo, Yuxin; Ji, Tianxiang; Long, Feng.
Afiliación
  • Chen D; School of Environment and Natural Resources, Renmin University of China, Beijing, 100872, China.
  • Xu W; School of Environment and Natural Resources, Renmin University of China, Beijing, 100872, China.
  • Lu Y; School of Environment and Natural Resources, Renmin University of China, Beijing, 100872, China.
  • Zhuo Y; School of Environment and Natural Resources, Renmin University of China, Beijing, 100872, China.
  • Ji T; School of Environment and Natural Resources, Renmin University of China, Beijing, 100872, China.
  • Long F; School of Environment and Natural Resources, Renmin University of China, Beijing, 100872, China. Electronic address: longf04@ruc.edu.cn.
Biosens Bioelectron ; 257: 116281, 2024 Aug 01.
Article en En | MEDLINE | ID: mdl-38677021
ABSTRACT
Environmental antibiotics and antibiotic resistance genes (ARGs) pose considerable threat to humans and animals; thus, the rapid and sensitive parallel detection of these pollutants from a single sample is urgently required. However, traditional multiplexed analytic technologies detect only one type of target (e.g., small molecules or nucleic acids) per assay. To address this issue, Evanescent wave Dual-color fluorescence Fiber-embedded Optofluidic Nanochip (EDFON) was fabricated by integrating a fiber-embedded optofluidic nanochip with evanescent wave dual-color fluorescence technology. The EDFON was used for the parallel quantitative detection of sulfamerazine (SMR) and MCR-1 with high sensitivity and specificity by combining a heterogeneous immunoassay with a homogenous hybridization chain reaction based on time-resolved effects. LODs of 0.032 µg/L and 35 pM was obtained for SMR and MCR-1, respectively, within 20 min. To our best knowledge, the EDFON is the first device for the simultaneous detection of two type of targets in each test, which is highly valuable to prevent the global threats of antibiotics and ARGs. Comparison with liquid chromatography-mass spectrometry showed a strong linear relationship (R2 = 0.998) for SMR pollution in the Qinghe River, with spiked SMR and MCR-1 negative surface and wastewater samples showing recovery rates of 91.8-113.4%. These results demonstrate the excellent accuracy and reliability of the EDFON, with features such as multi-analyte detection, field-deployment, and minimal-equipment, rendering it revolutionary for environmental monitoring, food safety, and medical diagnostics.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Contaminantes Químicos del Agua / Técnicas Biosensibles / Antibacterianos Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Contaminantes Químicos del Agua / Técnicas Biosensibles / Antibacterianos Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido