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miR-194-3p regulates epithelial-mesenchymal transition in embryonic epicardial cells via p120/ß-catenin signaling.
Acta Biochim Biophys Sin (Shanghai) ; 56(5): 717-729, 2024 05 25.
Article en En | MEDLINE | ID: mdl-38676398
ABSTRACT
The epicardium is integral to cardiac development and facilitates endogenous heart regeneration and repair. While miR-194-3p is associated with cellular migration and invasion, its impact on epicardial cells remains uncharted. In this work we use gain-of-function and loss-of-function methodologies to investigate the function of miR-194-3p in cardiac development. We culture embryonic epicardial cells in vitro and subject them to transforming growth factor ß (TGF-ß) treatment to induce epithelial-mesenchymal transition (EMT) and monitor miR-194-3p expression. In addition, the effects of miR-194-3p mimics and inhibitors on epicardial cell development and changes in EMT are investigated. To validate the binding targets of miR-194-3p and its ability to recover the target gene-phenotype, we produce a mutant vector p120-catenin-3'UTR-MUT. In epicardial cells, TGF-ß-induced EMT results in a notable overexpression of miR-194-3p. The administration of miR-194-3p mimics promotes EMT, which is correlated with elevated levels of mesenchymal markers. Conversely, miR-194-3p inhibitor attenuates EMT. Further investigations reveal a negative correlation between miR-194-3p and p120-catenin, which influences ß-catenin level in the cell adhesion pathway. The suppression of EMT caused by the miR-194-3p inhibitor is balanced by silencing of p120-catenin. In conclusion, miR-194-3p directly targets p120-catenin and modulates its expression, which in turn alters ß-catenin expression, critically influencing the EMT process in the embryonic epicardial cells via the cell adhesion mechanism.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pericardio / Transducción de Señal / MicroARNs / Beta Catenina / Cateninas / Transición Epitelial-Mesenquimal Límite: Animals Idioma: En Revista: Acta Biochim Biophys Sin (Shanghai) Asunto de la revista: BIOFISICA / BIOQUIMICA Año: 2024 Tipo del documento: Article Pais de publicación: China

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pericardio / Transducción de Señal / MicroARNs / Beta Catenina / Cateninas / Transición Epitelial-Mesenquimal Límite: Animals Idioma: En Revista: Acta Biochim Biophys Sin (Shanghai) Asunto de la revista: BIOFISICA / BIOQUIMICA Año: 2024 Tipo del documento: Article Pais de publicación: China