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26SCS-Loaded SilMA/Col Composite Sponge with Well-Arranged Layers Promotes Angiogenesis-Based Diabetic Wound Repair by Mediating Macrophage Inflammatory Response.
Luo, Pin; Liu, Wei; Ye, Zhangyao; Zhang, Yuyu; Zhang, Zekun; Yi, Jing; Zeng, Rong; Yang, Shenyu; Tu, Mei.
Afiliación
  • Luo P; College of Chemistry and Materials Science, Jinan University, Huangpu Road 601, Guangzhou 510632, China.
  • Liu W; Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Jinan University, Guangzhou 510632, China.
  • Ye Z; College of Chemistry and Materials Science, Jinan University, Huangpu Road 601, Guangzhou 510632, China.
  • Zhang Y; Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Jinan University, Guangzhou 510632, China.
  • Zhang Z; College of Chemistry and Materials Science, Jinan University, Huangpu Road 601, Guangzhou 510632, China.
  • Yi J; Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Jinan University, Guangzhou 510632, China.
  • Zeng R; College of Chemistry and Materials Science, Jinan University, Huangpu Road 601, Guangzhou 510632, China.
  • Yang S; Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Jinan University, Guangzhou 510632, China.
  • Tu M; College of Chemistry and Materials Science, Jinan University, Huangpu Road 601, Guangzhou 510632, China.
Molecules ; 29(8)2024 Apr 17.
Article en En | MEDLINE | ID: mdl-38675654
ABSTRACT
Diabetic wound healing is a significant clinical challenge because abnormal immune cells in the wound cause chronic inflammation and impair tissue regeneration. Therefore, regulating the behavior and function of macrophages may be conducive to improving treatment outcomes in diabetic wounds. Herein, sulfated chitosan (26SCS)-containing composite sponges (26SCS-SilMA/Col-330) with well-arranged layers and high porosity were constructed based on collagen and silk fibroin, aiming to induce an appropriate inflammatory response and promote angiogenesis. The results indicated that the ordered topological structure of composite sponges could trigger the pro-inflammatory response of Mφs in the early stage, and rapid release of 26SCS in the early and middle stages (within the concentration range of 1-3 mg/mL) induced a positive inflammatory response; initiated the pro-inflammatory reaction of Mφs within 3 days; shifted M1 Mφs to the M2 phenotype within 3-7 days; and significantly up-regulated the expression of two typical angiogenic growth factors, namely VEGF and PDGF-BB, on day 7, leading to rapid HUVEC migration and angiogenesis. In vivo data also demonstrated that on the 14th day after surgery, the 26SCS-SilMA/Col-330-implanted areas exhibited less inflammation, faster re-epithelialization, more abundant collagen deposition and a greater number of blood vessels in the skin tissue. The composite sponges with higher 26SCS contents (the (5.0) 26SCS-SilMA/Col-330 and the (7.5) 26SCS-SilMA/Col-330) could better orchestrate the phenotype and function of Mφs and facilitate wound healing. These findings highlight that the 26SCS-SilMA/Col-330 sponges developed in this work might have great potential as a novel dressing for the treatment of diabetic wounds.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cicatrización de Heridas / Neovascularización Fisiológica / Quitosano / Inflamación / Macrófagos Límite: Animals / Humans / Male Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cicatrización de Heridas / Neovascularización Fisiológica / Quitosano / Inflamación / Macrófagos Límite: Animals / Humans / Male Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza