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In Situ Preparation of Tannic Acid-Modified Poly(N-isopropylacrylamide) Hydrogel Coatings for Boosting Cell Response.
Xu, Jufei; Liu, Xiangzhe; Liang, Pengpeng; Yuan, Hailong; Yang, Tianyou.
Afiliación
  • Xu J; Department of Pharmacy, Air Force Medical Center, PLA, Air Force Medical University, Beijing 100142, China.
  • Liu X; School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003, China.
  • Liang P; School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003, China.
  • Yuan H; University of Chinese Academy of Sciences, Beijing 100049, China.
  • Yang T; School of Materials Science and Engineering, Zhengzhou University, Zhengzhou 450001, China.
Pharmaceutics ; 16(4)2024 Apr 13.
Article en En | MEDLINE | ID: mdl-38675199
ABSTRACT
The improvement of the capability of poly(N-isopropylacrylamide) (PNIPAAm) hydrogel coating in cell adhesion and detachment is critical to efficiently prepare cell sheets applied in cellular therapies and tissue engineering. To enhance cell response on the surface, the amine group-modified PNIPAAm (PNIPAAm-APTES) nanohydrogels were synthesized and deposited spontaneously on tannic acid (TA)-modified polyethylene (PE) plates. Subsequently, TA was introduced onto PNIPAAm-APTES nanohydrogels to fabricate coatings composed of TA-modified PNIPAAm-APTES (PNIPAAm-APTES-TA). Characterization techniques, including TEM, SEM, XPS, and UV-Vis spectroscopy, confirmed the effective deposition of hydrogels of PNIPAAm as well as the morphologies, content of chemical bonding-TA, and stability of various coatings. Importantly, the porous hydrogel coatings exhibited superhydrophilicity at 20 °C and thermo-responsive behavior. The fluorescence measurement demonstrated that the coating's stability effectively regulated protein behavior, influencing cell response. Notably, cell response tests revealed that even without precise control over the chain length/thickness of PNIPAAm during synthesis, the coatings enhanced cell adhesion and detachment, facilitating efficient cell culture. This work represented a novel and facile approach to preparing bioactive PNIPAAm for cell culture.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Pharmaceutics Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Pharmaceutics Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza