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Genomic and Phenotypic Analysis of Salmonella enterica Bacteriophages Identifies Two Novel Phage Species.
Bhandare, Sudhakar; Lawal, Opeyemi U; Colavecchio, Anna; Cadieux, Brigitte; Zahirovich-Jovich, Yella; Zhong, Zeyan; Tompkins, Elizabeth; Amitrano, Margot; Kukavica-Ibrulj, Irena; Boyle, Brian; Wang, Siyun; Levesque, Roger C; Delaquis, Pascal; Danyluk, Michelle; Goodridge, Lawrence.
Afiliación
  • Bhandare S; Food Safety and Quality Program, Department of Food Science and Agricultural Chemistry, McGill University, Montreal, QC H9X 3V9, Canada.
  • Lawal OU; School of Veterinary Medicine and Science, University of Nottingham, Nottingham LE12 5RD, UK.
  • Colavecchio A; Canadian Research Institute for Food Safety, Department of Food Science, University of Guelph, Guelph, ON N1G 2W1, Canada.
  • Cadieux B; Food Safety and Quality Program, Department of Food Science and Agricultural Chemistry, McGill University, Montreal, QC H9X 3V9, Canada.
  • Zahirovich-Jovich Y; Food Safety and Quality Program, Department of Food Science and Agricultural Chemistry, McGill University, Montreal, QC H9X 3V9, Canada.
  • Zhong Z; Food Safety and Quality Program, Department of Food Science and Agricultural Chemistry, McGill University, Montreal, QC H9X 3V9, Canada.
  • Tompkins E; Food Safety and Quality Program, Department of Food Science and Agricultural Chemistry, McGill University, Montreal, QC H9X 3V9, Canada.
  • Amitrano M; Food Safety and Quality Program, Department of Food Science and Agricultural Chemistry, McGill University, Montreal, QC H9X 3V9, Canada.
  • Kukavica-Ibrulj I; Food Safety and Quality Program, Department of Food Science and Agricultural Chemistry, McGill University, Montreal, QC H9X 3V9, Canada.
  • Boyle B; Institute for Integrative Systems Biology (IBIS), Laval University, Québec, QC G1V 0A6, Canada.
  • Wang S; Institute for Integrative Systems Biology (IBIS), Laval University, Québec, QC G1V 0A6, Canada.
  • Levesque RC; Faculty of Land and Food Systems, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.
  • Delaquis P; Institute for Integrative Systems Biology (IBIS), Laval University, Québec, QC G1V 0A6, Canada.
  • Danyluk M; Agriculture and Agri-Food Canada, Summerland, BC V0H 1Z0, Canada.
  • Goodridge L; Food Science and Human Nutrition Department, University of Florida, Gainesville, FL 32611, USA.
Microorganisms ; 12(4)2024 Mar 29.
Article en En | MEDLINE | ID: mdl-38674639
ABSTRACT
Bacteriophages (phages) are potential alternatives to chemical antimicrobials against pathogens of public health significance. Understanding the diversity and host specificity of phages is important for developing effective phage biocontrol approaches. Here, we assessed the host range, morphology, and genetic diversity of eight Salmonella enterica phages isolated from a wastewater treatment plant. The host range analysis revealed that six out of eight phages lysed more than 81% of the 43 Salmonella enterica isolates tested. The genomic sequences of all phages were determined. Whole-genome sequencing (WGS) data revealed that phage genome sizes ranged from 41 to 114 kb, with GC contents between 39.9 and 50.0%. Two of the phages SB13 and SB28 represent new species, Epseptimavirus SB13 and genera Macdonaldcampvirus, respectively, as designated by the International Committee for the Taxonomy of Viruses (ICTV) using genome-based taxonomic classification. One phage (SB18) belonged to the Myoviridae morphotype while the remaining phages belonged to the Siphoviridae morphotype. The gene content analyses showed that none of the phages possessed virulence, toxin, antibiotic resistance, type I-VI toxin-antitoxin modules, or lysogeny genes. Three (SB3, SB15, and SB18) out of the eight phages possessed tailspike proteins. Whole-genome-based phylogeny of the eight phages with their 113 homologs revealed three clusters A, B, and C and seven subclusters (A1, A2, A3, B1, B2, C1, and C2). While cluster C1 phages were predominantly isolated from animal sources, cluster B contained phages from both wastewater and animal sources. The broad host range of these phages highlights their potential use for controlling the presence of S. enterica in foods.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Microorganisms Año: 2024 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Microorganisms Año: 2024 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Suiza