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The Impact of Blood Sample Processing on Ribonucleic Acid (RNA) Sequencing.
Liu, Zhiyu; Ouyang, Tinglan; Yang, Yuwei; Sheng, Yuqi; Shi, Huajuan; Liu, Quanjun; Bai, Yunfei; Ge, Qinyu.
Afiliación
  • Liu Z; State Key Laboratory of Digital Medical Engineering, Southeast University, Nanjing 211189, China.
  • Ouyang T; State Key Laboratory of Digital Medical Engineering, Southeast University, Nanjing 211189, China.
  • Yang Y; State Key Laboratory of Digital Medical Engineering, Southeast University, Nanjing 211189, China.
  • Sheng Y; State Key Laboratory of Digital Medical Engineering, Southeast University, Nanjing 211189, China.
  • Shi H; State Key Laboratory of Digital Medical Engineering, Southeast University, Nanjing 211189, China.
  • Liu Q; State Key Laboratory of Digital Medical Engineering, Southeast University, Nanjing 211189, China.
  • Bai Y; State Key Laboratory of Digital Medical Engineering, Southeast University, Nanjing 211189, China.
  • Ge Q; State Key Laboratory of Digital Medical Engineering, Southeast University, Nanjing 211189, China.
Genes (Basel) ; 15(4)2024 04 17.
Article en En | MEDLINE | ID: mdl-38674435
ABSTRACT
In gene quantification and expression analysis, issues with sample selection and processing can be serious, as they can easily introduce irrelevant variables and lead to ambiguous results. This study aims to investigate the extent and mechanism of the impact of sample selection and processing on ribonucleic acid (RNA) sequencing. RNA from PBMCs and blood samples was investigated in this study. The integrity of this RNA was measured under different storage times. All the samples underwent high-throughput sequencing for comprehensive evaluation. The differentially expressed genes and their potential functions were analyzed after the samples were placed at room temperature for 0h, 4h and 8h, and different feature changes in these samples were also revealed. The sequencing results showed that the differences in gene expression were higher with an increased storage time, while the total number of genes detected did not change significantly. There were five genes showing gradient patterns over different storage times, all of which were protein-coding genes that had not been mentioned in previous studies. The effect of different storage times on seemingly the same samples was analyzed in this present study. This research, therefore, provides a theoretical basis for the long-term consideration of whether sample processing should be adequately addressed.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN / Análisis de Secuencia de ARN / Secuenciación de Nucleótidos de Alto Rendimiento Límite: Female / Humans / Male Idioma: En Revista: Genes (Basel) Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN / Análisis de Secuencia de ARN / Secuenciación de Nucleótidos de Alto Rendimiento Límite: Female / Humans / Male Idioma: En Revista: Genes (Basel) Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza