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Metabolic Comparison and Molecular Networking of Antimicrobials in Streptomyces Species.
Thapa, Bijaya Bahadur; Huo, Chen; Budhathoki, Rabin; Chaudhary, Pratiksha; Joshi, Soniya; Poudel, Purna Bahadur; Magar, Rubin Thapa; Parajuli, Niranjan; Kim, Ki Hyun; Sohng, Jae Kyung.
Afiliación
  • Thapa BB; Central Department of Chemistry, Tribhuvan University, Kirtipur 44618, Kathmandu, Nepal.
  • Huo C; School of Pharmacy, Sungkyunkwan University, Suwon 16419, Republic of Korea.
  • Budhathoki R; Central Department of Chemistry, Tribhuvan University, Kirtipur 44618, Kathmandu, Nepal.
  • Chaudhary P; Central Department of Chemistry, Tribhuvan University, Kirtipur 44618, Kathmandu, Nepal.
  • Joshi S; Central Department of Chemistry, Tribhuvan University, Kirtipur 44618, Kathmandu, Nepal.
  • Poudel PB; Institute of Biomolecule Reconstruction (iBR), Department of Life Science and Biochemical Engineering, Sun Moon University, Asan 31460, Republic of Korea.
  • Magar RT; Institute of Biomolecule Reconstruction (iBR), Department of Life Science and Biochemical Engineering, Sun Moon University, Asan 31460, Republic of Korea.
  • Parajuli N; Central Department of Chemistry, Tribhuvan University, Kirtipur 44618, Kathmandu, Nepal.
  • Kim KH; Institute of Biomolecule Reconstruction (iBR), Department of Life Science and Biochemical Engineering, Sun Moon University, Asan 31460, Republic of Korea.
  • Sohng JK; School of Pharmacy, Sungkyunkwan University, Suwon 16419, Republic of Korea.
Int J Mol Sci ; 25(8)2024 Apr 10.
Article en En | MEDLINE | ID: mdl-38673777
ABSTRACT
Streptomyces are well-known for producing bioactive secondary metabolites, with numerous antimicrobials essential to fight against infectious diseases. Globally, multidrug-resistant (MDR) microorganisms significantly challenge human and veterinary diseases. To tackle this issue, there is an urgent need for alternative antimicrobials. In the search for potent agents, we have isolated four Streptomyces species PC1, BT1, BT2, and BT3 from soils collected from various geographical regions of the Himalayan country Nepal, which were then identified based on morphology and 16S rRNA gene sequencing. The relationship of soil microbes with different Streptomyces species has been shown in phylogenetic trees. Antimicrobial potency of isolates was carried out against Staphylococcus aureus American Type Culture Collection (ATCC) 43300, Shigella sonnei ATCC 25931, Salmonella typhi ATCC 14028, Klebsiella pneumoniae ATCC 700603, and Escherichia coli ATCC 25922. Among them, Streptomyces species PC1 showed the highest zone of inhibition against tested pathogens. Furthermore, ethyl acetate extracts of shake flask fermentation of these Streptomyces strains were subjected to liquid chromatography-tandem mass spectrometric (LC-MS/MS) analysis for their metabolic comparison and Global Natural Products Social Molecular Networking (GNPS) web-based molecular networking. We found very similar metabolite composition in four strains, despite their geographical variation. In addition, we have identified thirty-seven metabolites using LC-MS/MS analysis, with the majority belonging to the diketopiperazine class. Among these, to the best of our knowledge, four metabolites, namely cyclo-(Ile-Ser), 2-n-hexyl-5-n-propylresorcinol, 3-[(6-methylpyrazin-2-yl) methyl]-1H-indole, and cyclo-(d-Leu-l-Trp), were detected for the first time in Streptomyces species. Besides these, other 23 metabolites including surfactin B, surfactin C, surfactin D, and valinomycin were identified with the help of GNPS-based molecular networking.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filogenia / Streptomyces Idioma: En Revista: Int J Mol Sci Año: 2024 Tipo del documento: Article País de afiliación: Nepal Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filogenia / Streptomyces Idioma: En Revista: Int J Mol Sci Año: 2024 Tipo del documento: Article País de afiliación: Nepal Pais de publicación: Suiza