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Uncovering Novel Protein Partners of Inducible Nitric Oxide Synthase in Human Testis.
Prabhakara, Karthik S; Ganapathy, Kavya; Islam, Kazi N; Thyagarajan, Hiran M; Tiwari, Kirti K; Parimi, Ramya L; Rashid, Mohammad B.
Afiliación
  • Prabhakara KS; Department of Biology and Biotechnology, College of Science and Engineering, University of Houston-Clear Lake, 2700 Bay Area Blvd., Houston, TX 77058-1098, USA.
  • Ganapathy K; Department of Biology and Biotechnology, College of Science and Engineering, University of Houston-Clear Lake, 2700 Bay Area Blvd., Houston, TX 77058-1098, USA.
  • Islam KN; Department of Agricultural Research and Development Program, Central State University, 1400 Brush Row Road, Wilberforce, OH 45384, USA.
  • Thyagarajan HM; Department of Biology and Biotechnology, College of Science and Engineering, University of Houston-Clear Lake, 2700 Bay Area Blvd., Houston, TX 77058-1098, USA.
  • Tiwari KK; Department of Biology and Biotechnology, College of Science and Engineering, University of Houston-Clear Lake, 2700 Bay Area Blvd., Houston, TX 77058-1098, USA.
  • Parimi RL; Department of Biology and Biotechnology, College of Science and Engineering, University of Houston-Clear Lake, 2700 Bay Area Blvd., Houston, TX 77058-1098, USA.
  • Rashid MB; Department of Biology and Biotechnology, College of Science and Engineering, University of Houston-Clear Lake, 2700 Bay Area Blvd., Houston, TX 77058-1098, USA.
Biomolecules ; 14(4)2024 Mar 24.
Article en En | MEDLINE | ID: mdl-38672406
ABSTRACT
Peroxidative damage to human spermatozoa has been shown to be the primary cause of male infertility. The possible role of nitric oxide (NO) in affecting sperm motility, capacitation, and acrosome reaction has been reported, too. The overproduction of NO by the enzyme inducible nitric oxide synthase (iNOS) could be responsible as it has been implicated in the pathogenesis of many diseases. There have been many studies on regulating iNOS function in various tissues, especially by protein-protein interaction; however, no study has looked for iNOS-interacting proteins in the human testis. Here, we have reported the identification of two proteins that interact with iNOS. We initially undertook a popular yeast two-hybrid assay to screen a human testis cDNA library in yeast using an iNOS-peptide fragment (amino acids 181-335) as bait. We verified our data using the mammalian chemiluminescent co-IP method; first, employing the same peptide and, then, a full-length protein co-expressed in HEK293 cells in addition to the candidate protein. In both cases, these two protein partners of iNOS were revealed (a) sperm acrosome-associated 7 protein and (b) retinoblastoma tumor-suppressor binding protein.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Testículo / Técnicas del Sistema de Dos Híbridos / Óxido Nítrico Sintasa de Tipo II Límite: Humans / Male Idioma: En Revista: Biomolecules Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Testículo / Técnicas del Sistema de Dos Híbridos / Óxido Nítrico Sintasa de Tipo II Límite: Humans / Male Idioma: En Revista: Biomolecules Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza