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Linkage Mapping and Genome-Wide Association Study Identified Two Peanut Late Leaf Spot Resistance Loci, PLLSR-1 and PLLSR-2, Using Nested Association Mapping.
Gangurde, Sunil S; Thompson, Ethan; Yaduru, Shasidhar; Wang, Hui; Fountain, Jake C; Chu, Ye; Ozias-Akins, Peggy; Isleib, Thomas G; Holbrook, Corley; Dutta, Bhabesh; Culbreath, Albert K; Pandey, Manish K; Guo, Baozhu.
Afiliación
  • Gangurde SS; U.S. Department of Agriculture, Agricultural Research Service, Crop Genetics and Breeding Research Unit, Tifton, GA, U.S.A.
  • Thompson E; Department of Plant Pathology, University of Georgia, Tifton, GA, U.S.A.
  • Yaduru S; Center of Excellence in Genomics & Systems Biology, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, Telangana, India.
  • Wang H; U.S. Department of Agriculture, Agricultural Research Service, Crop Genetics and Breeding Research Unit, Tifton, GA, U.S.A.
  • Fountain JC; Department of Plant Pathology, University of Georgia, Tifton, GA, U.S.A.
  • Chu Y; U.S. Department of Agriculture, Agricultural Research Service, Crop Genetics and Breeding Research Unit, Tifton, GA, U.S.A.
  • Ozias-Akins P; Center of Excellence in Genomics & Systems Biology, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, Telangana, India.
  • Isleib TG; U.S. Department of Agriculture, Agricultural Research Service, Crop Genetics and Breeding Research Unit, Tifton, GA, U.S.A.
  • Holbrook C; Department of Plant Pathology, University of Georgia, Tifton, GA, U.S.A.
  • Dutta B; Department of Plant Pathology, University of Georgia, Griffin, GA, U.S.A.
  • Culbreath AK; Department of Horticulture, University of Georgia, Tifton, GA, U.S.A.
  • Pandey MK; Department of Horticulture, University of Georgia, Tifton, GA, U.S.A.
  • Guo B; Department of Crop and Soil Sciences, North Carolina State University, Raleigh, NC, U.S.A.
Phytopathology ; 114(6): 1346-1355, 2024 Jun.
Article en En | MEDLINE | ID: mdl-38669464
ABSTRACT
Identification of candidate genes and molecular markers for late leaf spot (LLS) disease resistance in peanut (Arachis hypogaea) has been a focus of molecular breeding for the U.S. industry-funded peanut genome project. Efforts have been hindered by limited mapping resolution due to low levels of genetic recombination and marker density available in traditional biparental mapping populations. To address this, a multi-parental nested association mapping population has been genotyped with the peanut 58K single-nucleotide polymorphism (SNP) array and phenotyped for LLS severity in the field for 3 years. Joint linkage-based quantitative trait locus (QTL) mapping identified nine QTLs for LLS resistance with significant phenotypic variance explained up to 47.7%. A genome-wide association study identified 13 SNPs consistently associated with LLS resistance. Two genomic regions harboring the consistent QTLs and SNPs were identified from 1,336 to 1,520 kb (184 kb) on chromosome B02 and from 1,026.9 to 1,793.2 kb (767 kb) on chromosome B03, designated as peanut LLS resistance loci, PLLSR-1 and PLLSR-2, respectively. PLLSR-1 contains 10 nucleotide-binding site leucine-rich repeat disease resistance genes. A nucleotide-binding site leucine-rich repeat disease resistance gene, Arahy.VKVT6A, was also identified on homoeologous chromosome A02. PLLSR-2 contains five significant SNPs associated with five different genes encoding callose synthase, pollen defective in guidance protein, pentatricopeptide repeat, acyl-activating enzyme, and C2 GRAM domains-containing protein. This study highlights the power of multi-parent populations such as nested association mapping for genetic mapping and marker-trait association studies in peanuts. Validation of these two LLS resistance loci will be needed for marker-assisted breeding.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arachis / Enfermedades de las Plantas / Mapeo Cromosómico / Polimorfismo de Nucleótido Simple / Sitios de Carácter Cuantitativo / Estudio de Asociación del Genoma Completo / Resistencia a la Enfermedad Idioma: En Revista: Phytopathology Asunto de la revista: BOTANICA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arachis / Enfermedades de las Plantas / Mapeo Cromosómico / Polimorfismo de Nucleótido Simple / Sitios de Carácter Cuantitativo / Estudio de Asociación del Genoma Completo / Resistencia a la Enfermedad Idioma: En Revista: Phytopathology Asunto de la revista: BOTANICA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos