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Equinins as Novel Broad-Spectrum Antimicrobial Peptides Isolated from the Cnidarian Actinia equina (Linnaeus, 1758).
La Corte, Claudia; Catania, Valentina; Dara, Mariano; Parrinello, Daniela; Staropoli, Mariele; Trapani, Maria Rosa; Cammarata, Matteo; Parisi, Maria Giovanna.
Afiliación
  • La Corte C; Marine Immunobiology Laboratory, Department of Earth and Marine Sciences (DiSTeM), University of Palermo, Viale delle Scienze, Ed. 16, 90128 Palermo, Italy.
  • Catania V; NBFC-National Biodiversity Future Center, Piazza Marina 61, 90133 Palermo, Italy.
  • Dara M; NBFC-National Biodiversity Future Center, Piazza Marina 61, 90133 Palermo, Italy.
  • Parrinello D; Department of Earth and Marine Sciences (DiSTeM), University of Palermo, Viale delle Scienze, Ed. 16, 90128 Palermo, Italy.
  • Staropoli M; Marine Immunobiology Laboratory, Department of Earth and Marine Sciences (DiSTeM), University of Palermo, Viale delle Scienze, Ed. 16, 90128 Palermo, Italy.
  • Trapani MR; NBFC-National Biodiversity Future Center, Piazza Marina 61, 90133 Palermo, Italy.
  • Cammarata M; Marine Immunobiology Laboratory, Department of Earth and Marine Sciences (DiSTeM), University of Palermo, Viale delle Scienze, Ed. 16, 90128 Palermo, Italy.
  • Parisi MG; NBFC-National Biodiversity Future Center, Piazza Marina 61, 90133 Palermo, Italy.
Mar Drugs ; 22(4)2024 Apr 12.
Article en En | MEDLINE | ID: mdl-38667789
ABSTRACT
Sea anemones are valuable for therapeutic research as a diversified source of bioactive molecules, due to their diverse bioactive molecules linked to predation and defence mechanisms involving toxins and antimicrobial peptides. Acid extracts from Actinia equina tentacles and body were examined for antibacterial activity against Gram-positive, Gram-negative bacteria, and fungi. The peptide fractions showed interesting minimum inhibitory concentration (MIC) values (up to 0.125 µg/mL) against the tested pathogens. Further investigation and characterization of tentacle acid extracts with significant antimicrobial activity led to the purification of peptides through reverse phase chromatography on solid phase and HPLC. Broad-spectrum antimicrobial peptide activity was found in 40% acetonitrile fractions. The resulting peptides had a molecular mass of 2612.91 and 3934.827 Da and MIC ranging from 0.06 to 0.20 mg/mL. Sequencing revealed similarities to AMPs found in amphibians, fish, and Cnidaria, with anti-Gram+, Gram-, antifungal, candidacidal, anti-methicillin-resistant Staphylococcus aureus, carbapenemase-producing, vancomycin-resistant bacteria, and multi-drug resistant activity. Peptides 6.2 and 7.3, named Equinin A and B, respectively, were synthesized and evaluated in vitro towards the above-mentioned bacterial pathogens. Equinin B exerted interesting antibacterial activity (MIC and bactericidal concentrations of 1 mg/mL and 0.25 mg/mL, respectively) and gene organization supporting its potential in applied research.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pruebas de Sensibilidad Microbiana Límite: Animals Idioma: En Revista: Mar Drugs Asunto de la revista: BIOLOGIA / FARMACOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pruebas de Sensibilidad Microbiana Límite: Animals Idioma: En Revista: Mar Drugs Asunto de la revista: BIOLOGIA / FARMACOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza