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Antibiofilm and Immune-Modulatory Activity of Cannabidiol and Cannabigerol in Oral Environments-In Vitro Study.
Garzón, Hernan Santiago; Loaiza-Oliva, Manuela; Martínez-Pabón, María Cecilia; Puerta-Suárez, Jenniffer; Téllez Corral, Mayra Alexandra; Bueno-Silva, Bruno; Suárez, Daniel R; Díaz-Báez, David; Suárez, Lina J.
Afiliación
  • Garzón HS; Programa de Doctorado en Ingeniería, Facultad de Ingeniería, Pontificia Universidad Javeriana, Bogotá 110231, Colombia.
  • Loaiza-Oliva M; Laboratory of Oral Microbiology, Faculty of Dentistry, University of Antioquia, Medellín 050010, Colombia.
  • Martínez-Pabón MC; Laboratory of Oral Microbiology, Faculty of Dentistry, University of Antioquia, Medellín 050010, Colombia.
  • Puerta-Suárez J; Laboratory of Oral Microbiology, Faculty of Dentistry, University of Antioquia, Medellín 050010, Colombia.
  • Téllez Corral MA; Grupo Reproducción, Departamento de Obstetricia y Ginecología, Facultad de Medicina, Universidad de Antioquia, Medellín 050012, Colombia.
  • Bueno-Silva B; Centro de Investigaciones Odontológicas, Facultad de Odontología, Pontificia Universidad Javeriana, Bogotá 110231, Colombia.
  • Suárez DR; Department of Periodontology, Dental Research Division, Guarulhos University, Guarulhos 07023-070, Brazil.
  • Díaz-Báez D; Departamento de Biociências, Faculdade de Odontologia de Piracicaba, Universidade de Campinas (UNICAMP), Piracicaba 13414-903, Brazil.
  • Suárez LJ; Programa de Doctorado en Ingeniería, Facultad de Ingeniería, Pontificia Universidad Javeriana, Bogotá 110231, Colombia.
Antibiotics (Basel) ; 13(4)2024 Apr 09.
Article en En | MEDLINE | ID: mdl-38667018
ABSTRACT

OBJECTIVE:

To evaluate the in vitro antimicrobial and antibiofilm properties and the immune modulatory activity of cannabidiol (CBD) and cannabigerol (CBG) on oral bacteria and periodontal ligament fibroblasts (PLF).

METHODS:

Cytotoxicity was assessed by propidium iodide flow cytometry on fibroblasts derived from the periodontal ligament. The minimum inhibitory concentration (MIC) of CBD and CBG for S. mutans and C. albicans and the metabolic activity of a subgingival 33-species biofilm under CBD and CBG treatments were determined. The Quantification of cytokines was performed using the LEGENDplex kit (BioLegend, Ref 740930, San Diego, CA, USA).

RESULTS:

CBD-treated cell viability was greater than 95%, and for CBG, it was higher than 88%. MIC for S. mutans with CBD was 20 µM, and 10 µM for CBG. For C. albicans, no inhibitory effect was observed. Multispecies biofilm metabolic activity was reduced by 50.38% with CBD at 125 µg/mL (p = 0.03) and 39.9% with CBG at 62 µg/mL (p = 0.023). CBD exposure at 500 µg/mL reduced the metabolic activity of the formed biofilm by 15.41%, but CBG did not have an effect. CBG at 10 µM caused considerable production of anti-inflammatory mediators such as TGF-ß and IL-4 at 12 h. CBD at 10 µM to 20 µM produced the highest amount of IFN-γ.

CONCLUSION:

Both CBG and CBD inhibit S. mutans; they also moderately lower the metabolic activity of multispecies biofilms that form; however, CBD had an effect on biofilms that had already developed. This, together with the production of anti-inflammatory mediators and the maintenance of the viability of mammalian cells from the oral cavity, make these substances promising for clinical use and should be taken into account for future studies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Antibiotics (Basel) Año: 2024 Tipo del documento: Article País de afiliación: Colombia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Antibiotics (Basel) Año: 2024 Tipo del documento: Article País de afiliación: Colombia Pais de publicación: Suiza