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Enhanced TfR1 Recognition of Myocardial Injury after Acute Myocardial Infarction with Cardiac Fibrosis via Pre-Degrading Excess Fibrotic Collagen.
Yang, Wenwen; Wang, Yueqi; Li, Hongzheng; Liao, Feifei; Peng, Yuxuan; Lu, Aimei; Tan, Ling; Qu, Hua; Long, Linzi; Fu, Changgeng.
Afiliación
  • Yang W; Graduate School, China Academy of Chinese Medical Sciences, Beijing 100091, China.
  • Wang Y; CAS Key Laboratory of Molecular Imaging, Beijing Key Laboratory of Molecular Imaging, The State Key Laboratory of Management and Control for Complex Systems, Institute of Automation, Chinese Academy of Sciences, Beijing 100190, China.
  • Li H; CAS Key Laboratory of Molecular Imaging, Beijing Key Laboratory of Molecular Imaging, The State Key Laboratory of Management and Control for Complex Systems, Institute of Automation, Chinese Academy of Sciences, Beijing 100190, China.
  • Liao F; Graduate School, China Academy of Chinese Medical Sciences, Beijing 100091, China.
  • Peng Y; Graduate School, China Academy of Chinese Medical Sciences, Beijing 100091, China.
  • Lu A; Graduate School, China Academy of Chinese Medical Sciences, Beijing 100091, China.
  • Tan L; Graduate School, China Academy of Chinese Medical Sciences, Beijing 100091, China.
  • Qu H; Graduate School, China Academy of Chinese Medical Sciences, Beijing 100091, China.
  • Long L; Graduate School, China Academy of Chinese Medical Sciences, Beijing 100091, China.
  • Fu C; Graduate School, China Academy of Chinese Medical Sciences, Beijing 100091, China.
Biology (Basel) ; 13(4)2024 Mar 25.
Article en En | MEDLINE | ID: mdl-38666825
ABSTRACT
The fibrosis process after myocardial infarction (MI) results in a decline in cardiac function due to fibrotic collagen deposition and contrast agents' metabolic disorders, posing a significant challenge to conventional imaging strategies in making heart damage clear in the fibrosis microenvironment. To address this issue, we developed an imaging strategy. Specifically, we pretreated myocardial fibrotic collagen with collagenase I combined with human serum albumin (HSA-C) and subsequently visualized the site of cardiac injury by near-infrared (NIR) fluorescence imaging using an optical contrast agent (CI, CRT-indocyanine green) targeting transferrin receptor 1 peptides (CRT). The key point of this strategy is that pretreatment with HSA-C can reduce background signal interference in the fibrotic tissue while enhancing CI uptake at the heart lesion site, making the boundary between the injured heart tissue and the normal myocardium clearer. Our results showed that compared to that in the untargeted group, the normalized fluorescence intensity of cardiac damage detected by NIR in the targeted group increased 1.28-fold. The normalized fluorescence intensity increased 1.21-fold in the pretreatment group of the targeted groups. These data demonstrate the feasibility of applying pretreated fibrotic collagen and NIR contrast agents targeting TfR1 to identify ferroptosis at sites of cardiac injury, and its clinical value in the management of patients with MI needs further study.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biology (Basel) Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biology (Basel) Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza