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Serological and epidemiological investigation of Mycobacterium avium subspecies paratuberculosis in bovines in Pakistan.
Rehman, Aziz Ur; Javed, Muhammad Tariq; Ahmed, Ishtiaq; Saeed, Muhammad Adnan; Ehtisham-Ul-Haque, Syed; Rafique, Muhammad Kamran; Sikandar, Arbab; Nasir, Amar; Ahmad, Latif; Kashif, Muhammad; Zeeshan, Muhammad Abid.
Afiliación
  • Rehman AU; Department of Pathobiology (Pathology Section), University of Veterinary and Animal Sciences Lahore (Sub-Campus Jhang), Jhang, 35200, Pakistan.
  • Javed MT; Department of Pathology, Faculty of Veterinary Sciences, University of Agriculture, Faisalabad, 38000, Pakistan.
  • Ahmed I; Department of Pathobiology (Pathology Section), University of Veterinary and Animal Sciences Lahore (Sub-Campus Jhang), Jhang, 35200, Pakistan.
  • Saeed MA; Department of Pathobiology (Microbiology Section), University of Veterinary and Animal Sciences Lahore (Sub-Campus Jhang), Jhang, 35200, Pakistan.
  • Ehtisham-Ul-Haque S; Department of Pathobiology (Microbiology Section), University of Veterinary and Animal Sciences Lahore (Sub-Campus Jhang), Jhang, 35200, Pakistan.
  • Rafique MK; Department of Pathobiology (Pathology Section), University of Veterinary and Animal Sciences Lahore (Sub-Campus Jhang), Jhang, 35200, Pakistan.
  • Sikandar A; Department of Basic Sciences, University of Veterinary and Animal Sciences Lahore (Sub-Campus Jhang), Jhang, 35200, Pakistan.
  • Nasir A; Department of Clinical Sciences, University of Veterinary and Animal Sciences Lahore (Sub-Campus Jhang), Jhang, 35200, Pakistan.
  • Ahmad L; Baqai College of Veterinary Sciences, Baqai Medical University, Karachi, 74200, Pakistan.
  • Kashif M; Department of Clinical Sciences, University of Veterinary and Animal Sciences Lahore (Sub-Campus Jhang), Jhang, 35200, Pakistan.
  • Zeeshan MA; Department of Pathobiology (Pathology Section), University of Veterinary and Animal Sciences Lahore (Sub-Campus Jhang), Jhang, 35200, Pakistan.
Anim Biosci ; 37(9): 1644-1652, 2024 Sep.
Article en En | MEDLINE | ID: mdl-38665082
ABSTRACT

OBJECTIVE:

This study aimed to investigate the prevalence of paratuberculosis in cattle and buffaloes at twelve public dairy farms in Punjab, Pakistan.

METHODS:

A total of 2,181 more than two-year-old animals (1,242 cattle and 939 buffaloes) were tested by avian tuberculin, i.e., killed purified protein derivative of Mycobacterium avium paratuberculosis and indirect enzyme linked immunosorbent assay (ELISA). Blood and fecal samples were collected from tuberculin positive animals. These samples were further processed by indirect ELISA. The data were analyzed using frequency analysis and logistic analysis procedures.

RESULTS:

The prevalence of paratuberculosis at public dairy farms was 3.8%, as determined by tuberculin+ELISA test. It varied from 0.71% to 13.5% with a 100% herd prevalence. Multivariate logistic regression analysis revealed that species, milk production, total animals, total small ruminants, and total buffaloes were significantly associated with the occurrence of paratuberculosis. Odd ratio analysis revealed that with a one-kilogram increase in body weight, there will be a 0.006% increase in disease occurrence. With the increase in one animal in small ruminants and buffaloes, there will be 0.008% and 0.42% greater chances of developing paratuberculosis, respectively. Bivariate logistic regression analysis of cattle and buffaloes revealed that farm number, age, and total number of cattle were significantly associated with the occurrence of paratuberculosis. A one-month increase in lactation length increases the chance of tuberculosis by 0.004%; similarly, a one-liter increase in milk production increases the chance of disease by 10%. With each additional buffalo in the herd, there will be a 0.007% greater chance for the occurrence of paratuberculosis.

CONCLUSION:

This study concluded that tuberculin testing can be used in conjunction with ELISA to screen animals for paratuberculosis in countries with scarce resources, such as Pakistan. The efficacy of disease diagnosis can be improved by combining multiple tests.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Anim Biosci Año: 2024 Tipo del documento: Article País de afiliación: Pakistán Pais de publicación:
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Anim Biosci Año: 2024 Tipo del documento: Article País de afiliación: Pakistán Pais de publicación: