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Protective effects of antibiotic resistant bacteria on susceptibles in biofilm: Influential factors, mechanism, and modeling.
Xu, Fengqian; Jiang, Minxi; Li, Dan; Yu, Pingfeng; Ma, He; Lu, Huijie.
Afiliación
  • Xu F; Key Laboratory of Environment Remediation and Ecological Health, Ministry of Education, College of Environmental Resource Sciences, Zhejiang University, Hangzhou 310058, Zhejiang, China.
  • Jiang M; Department of Civil and Environmental Engineering, University of California, Berkeley, CA, USA.
  • Li D; Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Department of Environmental Science and Engineering, Fudan University, Shanghai 200438, China.
  • Yu P; Key Laboratory of Environment Remediation and Ecological Health, Ministry of Education, College of Environmental Resource Sciences, Zhejiang University, Hangzhou 310058, Zhejiang, China.
  • Ma H; Institute of Process Equipment, College of Energy Engineering, Zhejiang University, Hangzhou 310027, China.
  • Lu H; Key Laboratory of Environment Remediation and Ecological Health, Ministry of Education, College of Environmental Resource Sciences, Zhejiang University, Hangzhou 310058, Zhejiang, China. Electronic address: luhuijie@zju.edu.cn.
Sci Total Environ ; 930: 172668, 2024 Jun 20.
Article en En | MEDLINE | ID: mdl-38663625
ABSTRACT
In environmental biofilms, antibiotic-resistant bacteria facilitate the persistence of susceptible counterparts under antibiotic stresses, contributing to increased community-level resistance. However, there is a lack of quantitative understanding of this protective effect and its influential factors, hindering accurate risk assessment of biofilm resistance in diverse environment. This study isolated an opportunistic Escherichia coli pathogen from soil, and engineered it with plasmids conferring antibiotic resistance. Protective effects of the ampicillin resistant strain (AmpR) on their susceptible counterparts (AmpS) were observed in ampicillin-stress colony biofilms. The concentration of ampicillin delineated protective effects into 3 zones continuous protection (<1 MIC of AmpS), initial AmpS/R dependent (1-8 MIC of AmpS), and ineffective (>8 MIC of AmpS). Intriguingly, Zone 2 exhibited a surprising "less is more" phenomenon tuned by the initial AmpS/R ratio, where biofilm with an initially lower AmpR (150 vs 501) harbored 30-90 % more AmpR after 24 h growth under antibiotic stress. Compared to AmpS, AmpR displayed superiority in adhesion, antibiotic degradation, motility, and quorum sensing, allowing them to preferentially colonize biofilm edge and areas with higher ampicillin. An agent-based model incorporating protective effects successfully simulated tempo-spatial dynamics of AmpR and AmpS influenced by antibiotic stress and initial AmpS/R. This study provides a holistic view on the pervasive but poorly understood protective effects in biofilm, enabling development of better risk assessment and precisely targeted control strategies of biofilm resistance in diverse environment.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biopelículas / Escherichia coli / Antibacterianos Idioma: En Revista: Sci Total Environ Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biopelículas / Escherichia coli / Antibacterianos Idioma: En Revista: Sci Total Environ Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos