Your browser doesn't support javascript.
loading
Cooling rate modifies the location of aquaporin 3 in spermatozoa of sheep and goat.
Pequeño, Belén; Millán de la Blanca, María Gemma; Castaño, Cristina; Toledano-Díaz, Adolfo; Esteso, Milagros Cristina; Alba, Esther; Arrebola, Francisco A; Ungerfeld, Rodolfo; Martínez-Madrid, Belén; Alvarez-Rodriguez, Manuel; Rodriguez-Martinez, Heriberto; Santiago-Moreno, Julián.
Afiliación
  • Pequeño B; Dept. of Animal Reproduction, INIA-CSIC, Madrid, Spain.
  • Millán de la Blanca MG; Dept. of Animal Reproduction, INIA-CSIC, Madrid, Spain.
  • Castaño C; Dept. of Animal Reproduction, INIA-CSIC, Madrid, Spain.
  • Toledano-Díaz A; Dept. of Animal Reproduction, INIA-CSIC, Madrid, Spain.
  • Esteso MC; Dept. of Animal Reproduction, INIA-CSIC, Madrid, Spain.
  • Alba E; Dept. of Animal Reproduction, INIA-CSIC, Madrid, Spain.
  • Arrebola FA; Instituto Andaluz de Investigación y Formación Agraria, Pesquera y Alimentaria (IFAPA) Hinojosa Del Duque, Córdoba, Spain.
  • Ungerfeld R; Dept. Biociencias Veterinarias, Facultad de Veterinaria, Universidad de La República, Montevideo, Uruguay.
  • Martínez-Madrid B; Dept. Medicina y Cirugía Animal, Facultad de Veterinaria, UCM, Spain.
  • Alvarez-Rodriguez M; Dept. of Animal Reproduction, INIA-CSIC, Madrid, Spain.
  • Rodriguez-Martinez H; Dept. of Biomedical & Clinical Sciences, Obstetrics & Gynecology, Linköping University, Sweden.
  • Santiago-Moreno J; Dept. of Animal Reproduction, INIA-CSIC, Madrid, Spain. Electronic address: moreno@inia.csic.es.
Theriogenology ; 223: 29-35, 2024 Jul 15.
Article en En | MEDLINE | ID: mdl-38663138
ABSTRACT
The freeze-thawing process induces osmotic changes that may affect the membrane domain location of aquaporins' (AQP) in spermatozoa. Recent studies suggest that changes in AQP3 localization allows better sperm osmo-adaptation, improving the cryoresistance. Ultra-rapid freezing is an alternative cryopreservation technique that requires less equipment than conventional freezing, and it is faster, simpler and can be used in the field. This study aimed to determine the influence of freezing-thawing rates (slow (control) vs. ultra-rapid) on AQP3 expression and location in the spermatozoa from small ruminants (sheep and goats) and its relationship with sperm cryo-damage. Spermatozoa were collected from 10 Merino rams and 10 Murciano-Granadina bucks. The presence and distribution of AQP3 were assessed by Western blotting and immunocytochemistry (ICC), employing a commercial rabbit polyclonal antibody. Sperm motility was CASA system-analyzed, and membrane and acrosome integrity assessed by fluorescence (PI/PNA-FITC). Western blotting did not detect a significant effect of freezing-thawing rate on the amount of AQP3 while ICC found freezing-thawing rate affecting AQP3 location (P < 0.05). In both species, the percentages of spermatozoa showing AQP3 in the post-acrosome region, mid-piece, and principal piece of the tail were greater in samples cryopreserved by slow freezing-thawing (control) than ultra-rapid freezing-thawing rates (P < 0.05). Spermatozoa cryopreserved using ultra-rapid freezing-thawing showed decrease motility, plasma membrane, and acrosome integrity (P < 0.05), which might be related, at least in part, to a lower expression of AQP3. In conclusion, the cooling rate modifies the location of AQP3 in spermatozoa of sheep and goat, which might be associated with sperm cryosurvival.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Preservación de Semen / Espermatozoides / Cabras / Criopreservación / Acuaporina 3 Límite: Animals Idioma: En Revista: Theriogenology Año: 2024 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Preservación de Semen / Espermatozoides / Cabras / Criopreservación / Acuaporina 3 Límite: Animals Idioma: En Revista: Theriogenology Año: 2024 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos