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A novel entropy-driven dual-output mode integrated with DNAzyme for enhanced microRNA detection.
Zhang, Jianhong; Bai, Dan; Xie, Guoming; Xie, Yaxing; Lin, Yu; Hou, Yulei; Yu, Ying; Zhang, Yaoyi; Zhao, Rong; Wang, Zhongzhong; Wang, Luojia; Chen, Hui.
Afiliación
  • Zhang J; Clinical Laboratories, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, PR China.
  • Bai D; Key Laboratory of Laboratory Medical Diagnostics, Chinese Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, PR China.
  • Xie G; Key Laboratory of Laboratory Medical Diagnostics, Chinese Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, PR China.
  • Xie Y; Key Laboratory of Laboratory Medical Diagnostics, Chinese Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, PR China.
  • Lin Y; Clinical Laboratories, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, PR China.
  • Hou Y; Clinical Laboratories, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, PR China.
  • Yu Y; Clinical Laboratories, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, PR China.
  • Zhang Y; Key Laboratory of Laboratory Medical Diagnostics, Chinese Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, PR China.
  • Zhao R; Key Laboratory of Laboratory Medical Diagnostics, Chinese Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, PR China.
  • Wang Z; Key Laboratory of Laboratory Medical Diagnostics, Chinese Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, PR China.
  • Wang L; Key Laboratory of Laboratory Medical Diagnostics, Chinese Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, PR China.
  • Chen H; Clinical Laboratories, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, PR China. Electronic address: huichen@cqmu.edu.cn.
Talanta ; 275: 126123, 2024 Aug 01.
Article en En | MEDLINE | ID: mdl-38663065
ABSTRACT
Accurate microRNA (miRNA) detection is pivotal in the diagnosis and monitoring of cancer. Entropy-driven catalysis (EDC) has attracted widespread attention as an enzyme-free, isothermal technique for miRNA detection owing to its inherent simplicity and reliability. However, conventional EDC is a single-output mode, limiting the efficiency of signal amplification. In this study, a novel EDC dual-output mode was employed in conjunction with DNAzyme, resulting in the development of an EDC dual-end DNAzyme (EDC-DED) approach for highly sensitive miRNA detection. In this system, miRNA-21 initiated the EDC reaction, producing a large amount of catalytically active dual-end Mg2+-dependent DNAzyme. The DNAzyme further cleaved the reporter cyclically, generating a notably amplified fluorescence signal. The proposed method achieved a low detection limit of 2 pM. Compared with the traditional EDC single-end DNAzyme (EDC-SED) strategy, the present method exhibited superior amplification efficiency, enhancing detection sensitivity by approximately 46.5-fold. Furthermore, this platform demonstrated ideal specificity, satisfactory reproducibility and acceptable detection capabilities in clinical serum samples. Therefore, the straightforward and convenient strategy is a potential tool for miRNA analysis, which may provide a new perspective for biological analysis and clinical application.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Entropía / ADN Catalítico / MicroARNs Límite: Humans Idioma: En Revista: Talanta Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Entropía / ADN Catalítico / MicroARNs Límite: Humans Idioma: En Revista: Talanta Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos