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Dissolvable microneedles in the skin: Determination the impact of barrier disruption and dry skin on dissolution.
Limcharoen, Benchaphorn; Wanichwecharungruang, Supason; Kröger, Marius; Sansureerungsikul, Titiporn; Schleusener, Johannes; Lena Klein, Anna; Banlunara, Wijit; Meinke, Martina C; Darvin, Maxim E.
Afiliación
  • Limcharoen B; Department of Anatomy, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand; Center of Excellence in Advanced Materials and Biointerfaces, Chulalongkorn University, 10330, Thailand.
  • Wanichwecharungruang S; Department of Chemistry, Faculty of Science, Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand; Center of Excellence in Advanced Materials and Biointerfaces, Chulalongkorn University, 10330, Thailand.
  • Kröger M; Department of Dermatology, Venereology and Allergology, Center of Experimental and Applied Cutaneous Physiology (CCP), Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 10117, Germany.
  • Sansureerungsikul T; Mineed Technology, 928 Block 28, Building D, Chulalongkorn 7 Alley, Wangmai, Pathumwan, Bangkok 10330, Thailand.
  • Schleusener J; Department of Dermatology, Venereology and Allergology, Center of Experimental and Applied Cutaneous Physiology (CCP), Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 10117, Germany.
  • Lena Klein A; Department of Dermatology, Venereology and Allergology, Center of Experimental and Applied Cutaneous Physiology (CCP), Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 10117, Germany.
  • Banlunara W; Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand; Center of Excellence in Advanced Materials and Biointerfaces, Chulalongkorn University, 10330, Thailand.
  • Meinke MC; Department of Dermatology, Venereology and Allergology, Center of Experimental and Applied Cutaneous Physiology (CCP), Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 10117, Germany. Electronic address: martina.meinke@charite.de.
  • Darvin ME; Department of Dermatology, Venereology and Allergology, Center of Experimental and Applied Cutaneous Physiology (CCP), Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 10117, Germany. Electronic address: maxim.darvin@protonmail.co
Eur J Pharm Biopharm ; 199: 114303, 2024 Jun.
Article en En | MEDLINE | ID: mdl-38657740
ABSTRACT
Dissolvable microneedles (DMNs), fabricated from biocompatible materials that dissolve in both water and skin have gained popularity in dermatology. However, limited research exists on their application in compromised skin conditions. This study compares the hyaluronic acid-based DMNs penetration, formation of microchannels, dissolution, and diffusion kinetics in intact, barrier-disrupted (tape stripped), and dry (acetone-treated) porcine ear skin ex vivo. After DMNs application, comprehensive investigations including dermoscopy, stereomicroscope, skin hydration, transepidermal water loss (TEWL), optical coherence tomography (OCT), reflectance confocal laser scanning microscopy (RCLSM), confocal Raman micro-spectroscopy (CRM), two-photon tomography combined with fluorescence lifetime imaging (TPT-FLIM), histology, and scanning electron microscopy (SEM) were conducted. The 400 µm long DMNs successfully penetrated the skin to depths of ≈200 µm for dry skin and ≈200-290 µm for barrier-disrupted skin. Although DMNs fully inserted into all skin conditions, their dissolution rates were high in barrier-disrupted and low in dry skin, as observed through stereomicroscopy and TPT-FLIM. The dissolved polymer exhibited a more significant expansion in barrier-disrupted skin compared to intact skin, with the smallest increase observed in dry skin. Elevated TEWL and reduced skin hydration levels were evident in barrier-disrupted and dry skins compared to intact skin. OCT and RCLSM revealed noticeable skin indentation and pronounced microchannel areas, particularly in barrier-disrupted and dry skin. Additional confirmation of DMN effects on the skin and substance dissolution was obtained through histology, SEM, and CRM techniques. This study highlights the impact of skin condition on DMN effectiveness, emphasizing the importance of considering dissolvability and dissolution rates of needle materials, primarily composed of hyaluronic acid, for optimizing DMN-based drug delivery.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Piel / Absorción Cutánea / Solubilidad / Administración Cutánea / Ácido Hialurónico / Agujas Límite: Animals Idioma: En Revista: Eur J Pharm Biopharm Asunto de la revista: FARMACIA / FARMACOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Piel / Absorción Cutánea / Solubilidad / Administración Cutánea / Ácido Hialurónico / Agujas Límite: Animals Idioma: En Revista: Eur J Pharm Biopharm Asunto de la revista: FARMACIA / FARMACOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Países Bajos