Paucibacter sediminis sp. nov., isolated from sediment in a freshwater pond.
Int J Syst Evol Microbiol
; 74(4)2024 Apr.
Article
en En
| MEDLINE
| ID: mdl-38656473
ABSTRACT
A Gram-stain-negative, aerobic, oxidase-positive, weakly catalase-positive, motile by means of a single polar flagellum, rod-shaped bacterium designated as strain S2-9T was isolated from sediment sampled in Wiyang pond, Republic of Korea. Growth of this strain was observed at 10-40â°C (optimum, 35â°C) and pH 5.5-9.5 (optimum, pH 7.0-8.0) and in the presence of 0-0.5â% NaCl in Reasoner's 2A broth. The major fatty acids (>10â%) of strain S2-9T were C16ââ0 and summed feature 3 (comprising a mixture of C16ââ1 ω7c and/or C16ââ1 ω6c). Ubiquinone-8 was detected as the respiratory quinone. The major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. Strain S2-9T showed the highest 16S rRNA gene sequence similarity to Paucibacter oligotrophus CHU3T (98.7â%), followed by 'Paucibacter aquatile' CR182 (98.4â%), all type strains of Pelomonas species (98.1-98.3â%), Mitsuaria chitosanitabida NBRC 102408T (97.9â%), Kinneretia asaccharophila KIN192T (97.8â%), Mitsuaria chitinivorans HWN-4T (97.4â%), and Paucibacter toxinivorans 2C20T (97.4â%). Phylogenetic trees based on the 16S rRNA gene and whole-genome sequences showed that strain S2-9T formed a tight phylogenetic lineage with Paucibacter species (CHU3T, CR182, and 2C20T). Average nucleotide identity and digital DNA-DNA hybridization values between strain S2-9T and Paucibacter strains were 76.6-79.3% and 19.5-21.5â%, respectively. The genomic DNA G+C content of strain S2-9T was 68.3 mol%. Notably, genes responsible for both sulphur oxidation and reduction and denitrification were found in the genome of strain S2-9T, suggesting that strain S2-9T is involved in the nitrogen and sulphur cycles in pond ecosystems. Based on the polyphasic taxonomic results, strain S2-9T represents a novel species of the genus Paucibacter, for which the name Paucibacter sediminis sp. nov. is proposed. The type strain is S2-9T (=âKACC 22267T=âJCM 34541T).
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Texto completo:
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Filogenia
/
Composición de Base
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ADN Bacteriano
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ARN Ribosómico 16S
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Estanques
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Técnicas de Tipificación Bacteriana
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Ubiquinona
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Análisis de Secuencia de ADN
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Sedimentos Geológicos
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Ácidos Grasos
País/Región como asunto:
Asia
Idioma:
En
Revista:
Int J Syst Evol Microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
2024
Tipo del documento:
Article
Pais de publicación:
Reino Unido