Your browser doesn't support javascript.
loading
Impact of inflammatory preconditioning on murine microglial proteome response induced by focal ischemic brain injury.
Helbing, Dario Lucas; Haas, Fabienne; Cirri, Emilio; Rahnis, Norman; Dau, Therese Thuy Dung; Kelmer Sacramento, Erika; Oraha, Nova; Böhm, Leopold; Lajqi, Trim; Fehringer, Pascal; Morrison, Helen; Bauer, Reinhard.
Afiliación
  • Helbing DL; Institute of Molecular Cell Biology, Jena University Hospital, Friedrich Schiller University, Jena, Germany.
  • Haas F; Leibniz Institute on Aging, Fritz Lipmann Institute, Jena, Germany.
  • Cirri E; Department of Psychiatry and Psychotherapy, Jena University Hospital, Friedrich Schiller University Jena, Jena, Germany.
  • Rahnis N; Center for Intervention and Research on Adaptive and Maladaptive Brain Circuits Underlying Mental Health (C-I-R-C), Jena-Magdeburg-Halle, Jena, Germany.
  • Dau TTD; German Center for Mental Health (DZPG), Site Halle-Jena-Magdeburg, Jena, Germany.
  • Kelmer Sacramento E; Institute of Molecular Cell Biology, Jena University Hospital, Friedrich Schiller University, Jena, Germany.
  • Oraha N; Leibniz Institute on Aging, Fritz Lipmann Institute, Jena, Germany.
  • Böhm L; Leibniz Institute on Aging, Fritz Lipmann Institute, Jena, Germany.
  • Lajqi T; Leibniz Institute on Aging, Fritz Lipmann Institute, Jena, Germany.
  • Fehringer P; Leibniz Institute on Aging, Fritz Lipmann Institute, Jena, Germany.
  • Morrison H; Institute of Molecular Cell Biology, Jena University Hospital, Friedrich Schiller University, Jena, Germany.
  • Bauer R; Leibniz Institute on Aging, Fritz Lipmann Institute, Jena, Germany.
Front Immunol ; 15: 1227355, 2024.
Article en En | MEDLINE | ID: mdl-38655254
ABSTRACT
Preconditioning with lipopolysaccharide (LPS) induces neuroprotection against subsequent cerebral ischemic injury, mainly involving innate immune pathways. Microglia are resident immune cells of the central nervous system (CNS) that respond early to danger signals through memory-like differential reprogramming. However, the cell-specific molecular mechanisms underlying preconditioning are not fully understood. To elucidate the distinct molecular mechanisms of preconditioning on microglia, we compared these cell-specific proteomic profiles in response to LPS preconditioning and without preconditioning and subsequent transient focal brain ischemia and reperfusion, - using an established mouse model of transient focal brain ischemia and reperfusion. A proteomic workflow, based on isolated microglia obtained from mouse brains by cell sorting and coupled to mass spectrometry for identification and quantification, was applied. Our data confirm that LPS preconditioning induces marked neuroprotection, as indicated by a significant reduction in brain infarct volume. The established brain cell separation method was suitable for obtaining an enriched microglial cell fraction for valid proteomic analysis. The results show a significant impact of LPS preconditioning on microglial proteome patterns by type I interferons, presumably driven by the interferon cluster regulator proteins signal transducer and activator of transcription1/2 (STAT1/2).
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Lipopolisacáridos / Microglía / Proteoma / Proteómica Límite: Animals Idioma: En Revista: Front Immunol Año: 2024 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Lipopolisacáridos / Microglía / Proteoma / Proteómica Límite: Animals Idioma: En Revista: Front Immunol Año: 2024 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Suiza