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Real-Time Monitoring of Tyrosine Hydroxylase Activity with a Ratiometric Fluorescent Probe.
Yao, Jia-Yi; Li, Lu; Xu, Ji-Xuan; Liu, Yan-Hong; Shi, Jing; Yu, Xiao-Qi; Kong, Qing-Quan; Li, Kun.
Afiliación
  • Yao JY; Key Laboratory of Green Chemistry and Technology of Ministry of Education, College of Chemistry, Sichuan University, 29 Wangjiang Road, Chengdu 610064, P. R. China.
  • Li L; Orthopedic Department, Hospital of Chengdu Office of People's Government of Tibetan Autonomous Region, Sichuan University, Chengdu, Sichuan 610041, P. R. China.
  • Xu JX; Orthopedic Department, Hospital of Chengdu Office of People's Government of Tibetan Autonomous Region, Sichuan University, Chengdu, Sichuan 610041, P. R. China.
  • Liu YH; Key Laboratory of Green Chemistry and Technology of Ministry of Education, College of Chemistry, Sichuan University, 29 Wangjiang Road, Chengdu 610064, P. R. China.
  • Shi J; Orthopedic Department, Hospital of Chengdu Office of People's Government of Tibetan Autonomous Region, Sichuan University, Chengdu, Sichuan 610041, P. R. China.
  • Yu XQ; Key Laboratory of Green Chemistry and Technology of Ministry of Education, College of Chemistry, Sichuan University, 29 Wangjiang Road, Chengdu 610064, P. R. China.
  • Kong QQ; Asymmetric Synthesis and Chiral Technology Key Laboratory of Sichuan Province, Department of Chemistry, Xihua University, Chengdu 610039, P. R. China.
  • Li K; Orthopedic Department, Hospital of Chengdu Office of People's Government of Tibetan Autonomous Region, Sichuan University, Chengdu, Sichuan 610041, P. R. China.
Anal Chem ; 96(18): 7082-7090, 2024 05 07.
Article en En | MEDLINE | ID: mdl-38652135
ABSTRACT
Parkinson's disease (PD) represents the second most widespread neurodegenerative disease, and early monitoring and diagnosis are urgent at present. Tyrosine hydroxylase (TH) is a key enzyme for producing dopamine, the levels of which can serve as an indicator for assessing the severity and progression of PD. This renders the specific detection and visualization of TH a strategically vital way to meet the above demands. However, a fluorescent probe for TH monitoring is still missing. Herein, three rationally designed wash-free ratiometric fluorescent probes were proposed. Among them, TH-1 exhibited ideal photophysical properties and specific dual-channel bioimaging of TH activity in SH-SY5Y nerve cells. Moreover, the probe allowed for in vivo imaging of TH activity in zebrafish brain and living striatal slices of mice. Overall, the ratiometric fluorescent probe TH-1 could serve as a potential tool for real-time monitoring of PD in complex biosystems.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tirosina 3-Monooxigenasa / Pez Cebra / Colorantes Fluorescentes Límite: Animals / Humans Idioma: En Revista: Anal Chem Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tirosina 3-Monooxigenasa / Pez Cebra / Colorantes Fluorescentes Límite: Animals / Humans Idioma: En Revista: Anal Chem Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos