MicroRNA164e suppresses NAC100 transcription factor-mediated synthesis of seed storage proteins in chickpea.

Chakraborty, Anirban; Singh, Baljinder; Pandey, Vimal; Parida, Swarup K; Bhatia, Sabhyata

Chakraborty, Anirban; Singh, Baljinder; Pandey, Vimal; Parida, Swarup K; Bhatia, Sabhyata.

Afiliación

Chakraborty A; National Institute of Plant Genome Research, Aruna Asaf Ali Marg, PO Box No. 10531, New Delhi, 110067, India.
Singh B; National Institute of Plant Genome Research, Aruna Asaf Ali Marg, PO Box No. 10531, New Delhi, 110067, India.
Pandey V; National Institute of Plant Genome Research, Aruna Asaf Ali Marg, PO Box No. 10531, New Delhi, 110067, India.
Parida SK; National Institute of Plant Genome Research, Aruna Asaf Ali Marg, PO Box No. 10531, New Delhi, 110067, India.
Bhatia S; National Institute of Plant Genome Research, Aruna Asaf Ali Marg, PO Box No. 10531, New Delhi, 110067, India.

New Phytol ; 242(6): 2652-2668, 2024 Jun.

Article en En | MEDLINE | ID: mdl-38649769

ABSTRACT

ABSTRACT

Development of protein-enriched chickpea varieties necessitates an understanding of specific genes and key regulatory circuits that govern the synthesis of seed storage proteins (SSPs). Here, we demonstrated the novel involvement of Ca-miR164e-CaNAC100 in regulating SSP synthesis in chickpea. Ca-miRNA164e was significantly decreased during seed maturation, especially in high-protein accessions. The miRNA was found to directly target the transactivation conferring C-terminal region of a nuclear-localized transcription factor, CaNAC100 as revealed using RNA ligase-mediated-rapid amplification of cDNA ends and target mimic assays. The functional role of CaNAC100 was demonstrated through seed-specific overexpression (NACOE) resulting in significantly augmented seed protein content (SPC) consequential to increased SSP transcription. Further, NACOE lines displayed conspicuously enhanced seed weight but reduced numbers and yield. Conversely, a downregulation of CaNAC100 and SSP transcripts was evident in seed-specific overexpression lines of Ca-miR164e that culminated in significantly lowered SPC. CaNAC100 was additionally demonstrated to transactivate the SSP-encoding genes by directly binding to their promoters as demonstrated using electrophoretic mobility shift and dual-luciferase reporter assays. Taken together, our study for the first time established a distinct role of CaNAC100 in positively influencing SSP synthesis and its critical regulation by CamiR164e, thereby serving as an understanding that can be utilized for developing SPC-rich chickpea varieties.

Asunto(s)

Cicer; Regulación de la Expresión Génica de las Plantas; MicroARNs; Proteínas de Almacenamiento de Semillas; Factores de Transcripción; Secuencia de Bases; Cicer/genética; Cicer/crecimiento & desarrollo; Regulación de la Expresión Génica de las Plantas/genética; MicroARNs/genética; MicroARNs/metabolismo; Proteínas de Plantas/metabolismo; Proteínas de Plantas/genética; Plantas Modificadas Genéticamente; Regiones Promotoras Genéticas/genética; Proteínas de Almacenamiento de Semillas/metabolismo; Proteínas de Almacenamiento de Semillas/genética; Semillas/metabolismo; Semillas/genética; Factores de Transcripción/metabolismo; Factores de Transcripción/genética; Activación Transcripcional/genética

Palabras clave

NAC100; chickpea; microRNA164; seed protein content; seed storage proteins; transactivation

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Transcripción / Regulación de la Expresión Génica de las Plantas / Cicer / MicroARNs / Proteínas de Almacenamiento de Semillas Idioma: En Revista: New Phytol Asunto de la revista: BOTANICA Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Reino Unido

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