Your browser doesn't support javascript.
loading
An in vitro model for postoperative cranial nerve dysfunction and a proposed method of rehabilitation with N-acetylcysteine microparticles.
Kita, Ashley; Kedeshian, Katherine; Hong, Michelle; Hoffman, Larry.
Afiliación
  • Kita A; Department of Head and Neck Surgery, David Geffen School of Medicine at University of California Los Angeles, 10883 Le Conte Avenue, CHS 63-170, Los Angeles, CA, 90095, USA. akita@mednet.ucla.edu.
  • Kedeshian K; Department of Head and Neck Surgery, David Geffen School of Medicine at University of California Los Angeles, 10883 Le Conte Avenue, CHS 63-170, Los Angeles, CA, 90095, USA.
  • Hong M; Department of Head and Neck Surgery, David Geffen School of Medicine at University of California Los Angeles, 10883 Le Conte Avenue, CHS 63-170, Los Angeles, CA, 90095, USA.
  • Hoffman L; Department of Head and Neck Surgery, David Geffen School of Medicine at University of California Los Angeles, 10883 Le Conte Avenue, CHS 63-170, Los Angeles, CA, 90095, USA.
Eur Arch Otorhinolaryngol ; 281(7): 3805-3812, 2024 Jul.
Article en En | MEDLINE | ID: mdl-38649541
ABSTRACT

PURPOSE:

When operating near cranial motor nerves, transient postoperative weakness of target muscles lasting weeks to months is often observed. As nerves are typically intact at a procedure's completion, paresis is hypothesized to result from a combination of neurapraxia and axonotmesis. As both neurapraxia and axonotmesis involve Schwann cell injury and require remyelination, we developed an in vitro RSC96 Schwann cell model of injury using hydrogen peroxide (H2O2) to induce oxidative stress and investigated the efficacy of candidate therapeutic agents to promote RSC96 viability. As a first step in developing a long-term local administration strategy, the most promising of these agents was incorporated into sustained-release microparticles and investigated for bioactivity using this assay.

METHODS:

The concentration of H2O2 which reduced viability by 50% was determined to establish a standard for inducing oxidative stress in RSC96 cultures. Fresh cultures were then co-dosed with H2O2 and the potential therapeutics melatonin, N-acetylcysteine, resveratrol, and 4-aminopyridine. Schwann cell viability was evaluated and the most efficacious agent, N-acetylcysteine, was encapsulated into microparticles. Eluted samples of N-acetylcysteine from microparticles was evaluated for retained bioactivity.

RESULTS:

100 µM N-acetylcysteine improved the viability of Schwann cells dosed with H2O2. 100 µM Microparticle-eluted N-acetylcysteine also enhanced Schwann cell viability.

CONCLUSION:

We developed a Schwann cell culture model of iatrogenic nerve injury and used this to identify N-acetylcysteine as an agent to promote recovery. N-acetylcysteine was packaged into microparticles and demonstrated promise as a locally administrable agent to reduce oxidative stress in Schwann cells.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Acetilcisteína / Células de Schwann / Estrés Oxidativo / Peróxido de Hidrógeno Límite: Animals Idioma: En Revista: Eur Arch Otorhinolaryngol Asunto de la revista: OTORRINOLARINGOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Acetilcisteína / Células de Schwann / Estrés Oxidativo / Peróxido de Hidrógeno Límite: Animals Idioma: En Revista: Eur Arch Otorhinolaryngol Asunto de la revista: OTORRINOLARINGOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Alemania