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CircRNA CDR1as affects functional repair after spinal cord injury and regulates fibrosis through the SMAD pathway.
Wang, Wenzhao; Liu, Chang; He, Dong; Shi, Guidong; Song, Ping; Zhang, Boqing; Li, Tian; Wei, Jianlu; Jiang, Yunpeng; Ma, Liang.
Afiliación
  • Wang W; Department of Orthopedic, Qilu Hospital of Shandong University, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China; Department of Orthopedics, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
  • Liu C; Department of Neurosurgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
  • He D; Department of Neurosurgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China; Post-doctoral Scientific Research Workstation, Shandong Freda Biotech Co., Ltd, Jinan, Shandong, China; Department of Histology and Embryology, Cheeloo College of Medicin
  • Shi G; Department of Orthopedic, Qilu Hospital of Shandong University, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China.
  • Song P; Department of Orthopedics, West China Hospital, Sichuan University, Chengdu, Sichuan, China; National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, Sichuan, China.
  • Zhang B; National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, Sichuan, China.
  • Li T; School of Basic Medicine, Fourth Military Medical University, Xi'an, 710032, China.
  • Wei J; Department of Orthopedic, Qilu Hospital of Shandong University, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China. Electronic address: 18560089157@163.com.
  • Jiang Y; Department of Orthopedic, Qilu Hospital of Shandong University, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China. Electronic address: yunpeng_jiang@sina.cn.
  • Ma L; Department of Orthopedics, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China. Electronic address: 71002106@sdutcm.edu.cn.
Pharmacol Res ; 204: 107189, 2024 Jun.
Article en En | MEDLINE | ID: mdl-38649124
ABSTRACT
Spinal cord injury (SCI) is a complex problem in modern medicine. Fibroblast activation and fibroscarring after SCI impede nerve recovery. Non-coding RNA plays an important role in the progression of many diseases, but the study of its role in the progression of spinal fibrosis is still emerging. Here, we investigated the function of circular RNAs, specifically antisense to the cerebellar degeneration-related protein 1 (CDR1as), in spinal fibrosis and characterized its molecular mechanism and pathophysiology. The presence of CDR1as in the spinal cord was verified by sequencing and RNA expression assays. The effects of inhibition of CDR1as on scar formation, inflammation and nerve regeneration after spinal cord injury were investigated in vivo and in vitro. Further, gene expression of miR-7a-5p and protein expression of transforming Growth Factor Beta Receptor II (TGF-ßR2) were measured to evaluate their predicted interactions with CDR1as. The regulatory effects and activation pathways were subsequently verified by miR-7a-5p inhibitor and siCDR1as. These results indicate that CDR1as/miR-7a-5p/TGF-ßR2 interactions may exert scars and nerves functions and suggest potential therapeutic targets for treating spinal fibrotic diseases.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Traumatismos de la Médula Espinal / Fibrosis / Transducción de Señal / MicroARNs / ARN Largo no Codificante / ARN Circular Límite: Animals Idioma: En Revista: Pharmacol Res Asunto de la revista: FARMACOLOGIA Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Traumatismos de la Médula Espinal / Fibrosis / Transducción de Señal / MicroARNs / ARN Largo no Codificante / ARN Circular Límite: Animals Idioma: En Revista: Pharmacol Res Asunto de la revista: FARMACOLOGIA Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos