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Ultrasensitive Aptasensor for α-Amatoxin Detection Based on the DNA Tetrahedral Nanostructure Triggering Rolling Circle Amplification and Signal Amplification.
Tian, Run; Sun, Jiadi; Ye, Yongli; Lu, Xin; Wang, Weiya; Sun, Xiulan.
Afiliación
  • Tian R; School of Food Science and Technology, International Joint Laboratory on Food Safety, Synergetic Innovation Center of Food Safety and Quality Control, Jiangnan University, Wuxi, Jiangsu 214122, P.R. China.
  • Sun J; Yixing Institute of Food and Biotechnology Co., Ltd., Yixing 214200, China.
  • Ye Y; School of Food Science and Technology, International Joint Laboratory on Food Safety, Synergetic Innovation Center of Food Safety and Quality Control, Jiangnan University, Wuxi, Jiangsu 214122, P.R. China.
  • Lu X; Yixing Institute of Food and Biotechnology Co., Ltd., Yixing 214200, China.
  • Wang W; School of Food Science and Technology, International Joint Laboratory on Food Safety, Synergetic Innovation Center of Food Safety and Quality Control, Jiangnan University, Wuxi, Jiangsu 214122, P.R. China.
  • Sun X; Yixing Institute of Food and Biotechnology Co., Ltd., Yixing 214200, China.
J Agric Food Chem ; 72(17): 10046-10054, 2024 May 01.
Article en En | MEDLINE | ID: mdl-38648503
ABSTRACT
Poisonous mushrooms containing α-amatoxin can be lethal, making it imperative to develop a rapid and sensitive detection method for α-amatoxin. Utilizing the DNA tetrahedral structure as its foundation, the aptamer allows controlled density and orientation. Consequently, we designed aptamer tetrahedral functionalized magnetic beads that specifically target α-amanitin to release complementary DNA (C-DNA) strands. These strands were then employed as primers to initiate rolling circle amplification (RCA) with fluorescent dyes. The combination of SYBR Green I detection probes facilitated the amplification of the detection signal, enhancing the detection sensitivity of the aptasensor. The calculated detection limit was determined to be 3 ng/mL, a magnitude lower than that of other aptasensors by 2 orders of magnitude. The aptasensor integrates the advantages of high sensitivity and specificity, offering a simple and reliable rapid detection method for α-amanitin analysis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Técnicas de Amplificación de Ácido Nucleico / Aptámeros de Nucleótidos / Límite de Detección Idioma: En Revista: J Agric Food Chem Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Técnicas de Amplificación de Ácido Nucleico / Aptámeros de Nucleótidos / Límite de Detección Idioma: En Revista: J Agric Food Chem Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos