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Increased paclitaxel recovery from Taxus baccata vascular stem cells using novel in situ product recovery approaches.
Santoyo-Garcia, Jorge H; Valdivia-Cabrera, Marissa; Ochoa-Villarreal, Marisol; Casasola-Zamora, Samuel; Ripoll, Magdalena; Escrich, Ainoa; Moyano, Elisabeth; Betancor, Lorena; Halliday, Karen J; Loake, Gary J; Rios-Solis, Leonardo.
Afiliación
  • Santoyo-Garcia JH; Institute for Bioengineering, School of Engineering, University of Edinburgh, King's Buildings, Edinburgh, EH9 3FB, UK. jorge.santoyogarcia@roslintech.com.
  • Valdivia-Cabrera M; Centre for Engineering Biology, University of Edinburgh, King's Buildings, Edinburgh, EH9 3BF, UK. jorge.santoyogarcia@roslintech.com.
  • Ochoa-Villarreal M; Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, King's Buildings, Edinburgh, EH9 3BF, UK.
  • Casasola-Zamora S; Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, King's Buildings, Edinburgh, EH9 3BF, UK.
  • Ripoll M; Green Bioactives, Douglas House, Pentland Science Park, Midlothian, EH16 0PL, UK.
  • Escrich A; Laboratorio de Biotecnología, Universidad ORT Uruguay, Mercedes 1237, 11100, Montevideo, Uruguay.
  • Moyano E; Graduate Program in Chemistry, Facultad de Química, Universidad de la República, Montevideo, Uruguay.
  • Betancor L; Department of Medicine and Life Sciences, Universitat Pompeu Fabra, 08003, Barcelona, Spain.
  • Halliday KJ; Department of Medicine and Life Sciences, Universitat Pompeu Fabra, 08003, Barcelona, Spain.
  • Loake GJ; Laboratorio de Biotecnología, Universidad ORT Uruguay, Mercedes 1237, 11100, Montevideo, Uruguay.
  • Rios-Solis L; Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, King's Buildings, Edinburgh, EH9 3BF, UK.
Bioresour Bioprocess ; 10(1): 68, 2023 Sep 29.
Article en En | MEDLINE | ID: mdl-38647629
ABSTRACT
In this study, several approaches were tested to optimise the production and recovery of the widely used anticancer drug Taxol® (paclitaxel) from culturable vascular stem cells (VSCs) of Taxus baccata, which is currently used as a successful cell line for paclitaxel production. An in situ product recovery (ISPR) technique was employed, which involved combining three commercial macro-porous resin beads (HP-20, XAD7HP and HP-2MG) with batch and semi-continuous cultivations of the T. baccata VSCs after adding methyl jasmonate (Me-JA) as an elicitor. The optimal resin combination resulted in 234 ± 23 mg of paclitaxel per kg of fresh-weight cells, indicating a 13-fold improved yield compared to the control (with no resins) in batch cultivation. This resin treatment was further studied to evaluate the resins' removal capacity of reactive oxygen species (ROS), which can cause poor cell growth or reduce product synthesis. It was observed that the ISPR cultivations had fourfold less intracellular ROS concentration than that of the control; thus, a reduced ROS concentration established by the resin contributed to increased paclitaxel yield, contrary to previous studies. These paclitaxel yields are the highest reported to date using VSCs, and this scalable production method could be applied for a diverse range of similar compounds utilising plant cell culture.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Bioresour Bioprocess Año: 2023 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Bioresour Bioprocess Año: 2023 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Alemania