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Simple and Rapid Method of Microwell Array Fabrication for Drug Testing on 3D Cancer Spheroids.
Nguyen, Mai Anh; Dinh, Nhung Thi; Do Thi, My Hanh; Nguyen Thi, Dung; Pham, Uyen Thu; Tran, Toan Quoc; Nguyen, Vuong Minh; Le, Nhung Hong; Nguyen, Duong Thanh; Pham, Dung Thuy Nguyen.
Afiliación
  • Nguyen MA; Institute for Tropical Technology, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet st., Cau Giay dist., Hanoi 100000, Vietnam.
  • Dinh NT; Hanoi University of Science and Technology (HUST), 1 Dai Co Viet st., Hai Ba Trung dist., Hanoi 100000, Vietnam.
  • Do Thi MH; Institute for Tropical Technology, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet st., Cau Giay dist., Hanoi 100000, Vietnam.
  • Nguyen Thi D; Institute for Tropical Technology, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet st., Cau Giay dist., Hanoi 100000, Vietnam.
  • Pham UT; Institute for Tropical Technology, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet st., Cau Giay dist., Hanoi 100000, Vietnam.
  • Tran TQ; University of Science and Technology of Hanoi (USTH), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet st., Cau Giay dist., Hanoi 100000, Vietnam.
  • Nguyen VM; Graduate University of Science and Technology, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet st., Cau Giay dist., Hanoi 100000, Vietnam.
  • Le NH; Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet St., Cau Giay Dist., Hanoi 100000, Vietnam.
  • Nguyen DT; Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet St., Cau Giay Dist., Hanoi 100000, Vietnam.
  • Pham DTN; Graduate University of Science and Technology, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet st., Cau Giay dist., Hanoi 100000, Vietnam.
ACS Omega ; 9(15): 16949-16958, 2024 Apr 16.
Article en En | MEDLINE | ID: mdl-38645317
ABSTRACT
Three-dimensional (3D) cell culture systems are becoming increasingly popular due to their ability to mimic the complex process of angiogenesis in cancer, providing more accurate and physiologically relevant data than traditional two-dimensional (2D) cell culture systems. Microwell systems are particularly useful in this context as they provide a microenvironment that more closely resembles the in vivo environment than traditional microwells. Poly(ethylene glycol) (PEG) microwells are particularly advantageous due to their bio-inertness and the ability to tailor their material characteristics depending on the PEG molecular weight. Although there are several methods available for microwell fabrication, most of them are time-consuming and expensive. The current study utilizes a low-cost laser etching technique on poly(methyl methacrylate) materials followed by molding with PDMS to produce microwells. The optimal conditions for making concave microwells are an engraving parameter speed of 600 mm/s, power of 20%, and a design diameter of the microwell of 0.4 mm. The artificial tumor achieved its full size after 7 days of cell growth in a microwell system, and the cells developed drugs through a live/dead assay test. The results of the drug testing revealed that the IC50 value of zerumbone-loaded liposomes in HepG2 was 4.53 pM, which is greater than the IC50 value of zerumbone. The HepG2 cancer sphere's 3D platform for medication testing revealed that zerumbone-loaded liposomes were very effective at high doses. These findings generally imply that zerumbone-loaded liposomes have the capacity to target the liver and maintain medication delivery.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: ACS Omega Año: 2024 Tipo del documento: Article País de afiliación: Vietnam Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: ACS Omega Año: 2024 Tipo del documento: Article País de afiliación: Vietnam Pais de publicación: Estados Unidos