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Cell-based tissue engineered flexor tendon allograft: A canine in vivo study.
Lin, Subin; Reisdorf, Ramona; Lu, Chun Kuan; Wang, Zhanwen; An, Kai-Nan; Moran, Steven L; Amadio, Peter C; Zhao, Chunfeng.
Afiliación
  • Lin S; Department of Orthopedic Surgery, Mayo Clinic, Rochester, Minnesota, USA.
  • Reisdorf R; Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.
  • Lu CK; Department of Orthopedic Surgery, Mayo Clinic, Rochester, Minnesota, USA.
  • Wang Z; Department of Orthopedic Surgery, Mayo Clinic, Rochester, Minnesota, USA.
  • An KN; Department of Orthopedic Surgery, Mayo Clinic, Rochester, Minnesota, USA.
  • Moran SL; Department of Orthopedic Surgery, Mayo Clinic, Rochester, Minnesota, USA.
  • Amadio PC; Department of Orthopedic Surgery, Mayo Clinic, Rochester, Minnesota, USA.
  • Zhao C; Department of Orthopedic Surgery, Mayo Clinic, Rochester, Minnesota, USA.
J Orthop Res ; 42(9): 1923-1932, 2024 Sep.
Article en En | MEDLINE | ID: mdl-38639414
ABSTRACT
This study aimed to compare the clinically established autologous extrasynovial tendon graft to a newly developed tissue-engineered allograft (Eng-allograft) in terms of functional outcomes following flexor tendon reconstruction in a canine model. The second and fifth flexor digitorum profundus (FDP) tendons from 16 dogs were transected and repaired in Zone II. After 6 weeks of cage activity, the repaired tendons were intentionally ruptured, creating a clinically relevant model for reconstruction. The re-ruptured FDP tendons were then reconstructed using either the clinically standard autologous extrasynovial tendon graft or the Eng-allograft, which had been revitalized with autologous bone marrow-derived mesenchymal stem cells (BMSCs) and synovialized using carbodiimide derivatized synovial fluid (cd-SYN). Following 12 weeks of postoperative rehabilitation, the functional outcomes of the surgical digits were evaluated. The Eng-allograft group exhibited improved digital function, including lower digit work of flexion and reduced adhesion status, while maintaining similar tendon gliding resistance compared to the autograft group. However, the failure load of both the distal and proximal host/graft conjunctions in the Eng-allograft group was significantly lower than that of the autograft group with higher graft rupture at the host-graft junction. In conclusion, the decellularized allogenic intrasynovial tendon, when revitalized BMSCs and synovialized with cd-SYN, demonstrates positive effects on digital function improvement and adhesion reduction. However, the healing at both proximal and distal graft/host junctions is far lower than the autograft. Further research is needed to enhance the healing capacity of allograft conjunctions, aiming to achieve a comparable level of healing seen with autografts.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tendones / Ingeniería de Tejidos / Aloinjertos Límite: Animals Idioma: En Revista: J Orthop Res Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tendones / Ingeniería de Tejidos / Aloinjertos Límite: Animals Idioma: En Revista: J Orthop Res Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos