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Plasmon-enhanced fluorescence combined with aptamer sensor based on Ag nanocubes for signal-amplified detection of berberine hydrochloride.
Chen, Shilin; Xu, Chenye; Zhu, Xingzhen; Li, Zhenghua; Bie, Haoran; Yang, Yang; Yu, Jingtian; Yang, Yaqiong; Huang, He.
Afiliación
  • Chen S; College of Food and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, 210023, PR China.
  • Xu C; College of Food and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, 210023, PR China.
  • Zhu X; College of Food and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, 210023, PR China.
  • Li Z; College of Food and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, 210023, PR China.
  • Bie H; College of Food and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, 210023, PR China.
  • Yang Y; College of Food and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, 210023, PR China.
  • Yu J; College of Food and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, 210023, PR China.
  • Yang Y; College of Food and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, 210023, PR China. Electronic address: yangyq@njnu.edu.cn.
  • Huang H; College of Food and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, 210023, PR China. Electronic address: huangh@njnu.edu.cn.
Anal Chim Acta ; 1304: 342579, 2024 May 22.
Article en En | MEDLINE | ID: mdl-38637044
ABSTRACT
Plasmon enhanced fluorescent (PEF) with more "hot spots" play a critical role in signal amplified technology to avoid the intrinsic limitation of fluorophore which ascribed to a strong electromagnetic field at the tip structure. However, application of PEF technique to obtain a highly sensitive analysis of medicine was still at a very early stage. Herein, a simple but versatile Ag nanocubes (Agcubes)-based PEF sensor combined with aptamer (Agcubes@SiO2-QDs-Apt) was proposed for highly sensitive detection of berberine hydrochloride (BH). The distance between the plasma Agcubes and the red-emitted CdTe quantum dots (QDs) were regulated by the thickness of silica spacer. The three-dimensional finite-difference time-domain (3D-FDTD) simulation further revealed that Agcubes have a higher electromagnetic field than Ag nanospheres. Compared with PEF sensor, signal QDs-modified aptamer without Agcubes (QDs-Apt) showed a 10-fold higher detection limit. The linear range and detection limit of the Agcubes@SiO2-QDs-Apt were 0.1-100 µM, 87.3 nM, respectively. Furthermore, the PEF sensor was applied to analysis BH in the berberine hydrochloride tablets, compound berberine tablet and urine with good recoveries of 98.25-102.05%. These results demonstrated that the prepared PEF sensor has great potential for drug quality control and clinical analysis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Berberina / Compuestos de Cadmio / Puntos Cuánticos / Aptámeros de Nucleótidos Idioma: En Revista: Anal Chim Acta Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Berberina / Compuestos de Cadmio / Puntos Cuánticos / Aptámeros de Nucleótidos Idioma: En Revista: Anal Chim Acta Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos