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Environmentally dependent and independent control of 3D cell shape.
Dent, Lucas G; Curry, Nathan; Sparks, Hugh; Bousgouni, Vicky; Maioli, Vincent; Kumar, Sunil; Munro, Ian; Butera, Francesca; Jones, Ian; Arias-Garcia, Mar; Rowe-Brown, Leo; Dunsby, Chris; Bakal, Chris.
Afiliación
  • Dent LG; Dynamical Cell Systems Group, Division of Cancer Biology, Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK.
  • Curry N; Photonics Group, Department of Physics, Imperial College London, London SW7 2AZ, UK.
  • Sparks H; Photonics Group, Department of Physics, Imperial College London, London SW7 2AZ, UK.
  • Bousgouni V; Dynamical Cell Systems Group, Division of Cancer Biology, Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK.
  • Maioli V; Photonics Group, Department of Physics, Imperial College London, London SW7 2AZ, UK.
  • Kumar S; Photonics Group, Department of Physics, Imperial College London, London SW7 2AZ, UK.
  • Munro I; Photonics Group, Department of Physics, Imperial College London, London SW7 2AZ, UK.
  • Butera F; Dynamical Cell Systems Group, Division of Cancer Biology, Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK.
  • Jones I; Dynamical Cell Systems Group, Division of Cancer Biology, Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK.
  • Arias-Garcia M; Dynamical Cell Systems Group, Division of Cancer Biology, Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK.
  • Rowe-Brown L; Photonics Group, Department of Physics, Imperial College London, London SW7 2AZ, UK.
  • Dunsby C; Photonics Group, Department of Physics, Imperial College London, London SW7 2AZ, UK. Electronic address: christopher.dunsby@imperial.ac.uk.
  • Bakal C; Dynamical Cell Systems Group, Division of Cancer Biology, Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK. Electronic address: chris.bakal@icr.ac.uk.
Cell Rep ; 43(5): 114016, 2024 May 28.
Article en En | MEDLINE | ID: mdl-38636520
ABSTRACT
How cancer cells determine their shape in response to three-dimensional (3D) geometric and mechanical cues is unclear. We develop an approach to quantify the 3D cell shape of over 60,000 melanoma cells in collagen hydrogels using high-throughput stage-scanning oblique plane microscopy (ssOPM). We identify stereotypic and environmentally dependent changes in shape and protrusivity depending on whether a cell is proximal to a flat and rigid surface or is embedded in a soft environment. Environmental sensitivity metrics calculated for small molecules and gene knockdowns identify interactions between the environment and cellular factors that are important for morphogenesis. We show that the Rho guanine nucleotide exchange factor (RhoGEF) TIAM2 contributes to shape determination in environmentally independent ways but that non-muscle myosin II, microtubules, and the RhoGEF FARP1 regulate shape in ways dependent on the microenvironment. Thus, changes in cancer cell shape in response to 3D geometric and mechanical cues are modulated in both an environmentally dependent and independent fashion.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Intercambio de Guanina Nucleótido / Forma de la Célula Límite: Humans Idioma: En Revista: Cell Rep Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Intercambio de Guanina Nucleótido / Forma de la Célula Límite: Humans Idioma: En Revista: Cell Rep Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos