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Purification of DNA Nanoparticles Using Photocleavable Biotin Tethers.
Everson, Heather R; Neyra, Kayla; Scarton, Dylan V; Chandrasekhar, Soumya; Green, Christopher M; Schmidt, Thorsten-Lars; Medintz, Igor L; Veneziano, Remi; Mathur, Divita.
Afiliación
  • Everson HR; Department of Chemistry, Case Western Reserve University, Cleveland, Ohio 44106, United States.
  • Neyra K; Department of Chemistry, Case Western Reserve University, Cleveland, Ohio 44106, United States.
  • Scarton DV; College of Science, Interdisciplinary Program in Neuroscience, George Mason University, Fairfax, Virginia 22030, United States.
  • Chandrasekhar S; Institute for Advanced Biomedical Research, George Mason University, Manassas, Virginia 20110, United States.
  • Green CM; Department of Physics, Kent State University, Kent, Ohio 44240, United States.
  • Schmidt TL; Center for Bio/Molecular Science and Engineering, US Naval Research Laboratory, Washington, District of Columbia 20375, United States.
  • Medintz IL; Department of Physics, Kent State University, Kent, Ohio 44240, United States.
  • Veneziano R; Center for Bio/Molecular Science and Engineering, US Naval Research Laboratory, Washington, District of Columbia 20375, United States.
  • Mathur D; Institute for Advanced Biomedical Research, George Mason University, Manassas, Virginia 20110, United States.
ACS Appl Mater Interfaces ; 16(17): 22334-22343, 2024 May 01.
Article en En | MEDLINE | ID: mdl-38635042
ABSTRACT
The number of applications of self-assembled deoxyribonucleic acid (DNA) origami nanoparticles (DNA NPs) has increased drastically, following the development of a variety of single-stranded template DNA (ssDNA) that can serve as the scaffold strand. In addition to viral genomes, such as M13 bacteriophage and lambda DNAs, enzymatically produced ssDNA from various template sources is rapidly gaining traction and being applied as the scaffold for DNA NP preparation. However, separating fully formed DNA NPs that have custom scaffolds from crude assembly mixes is often a multistep process of first separating the ssDNA scaffold from its enzymatic amplification process and then isolating the assembled DNA NPs from excess precursor strands. Only then is the DNA NP sample ready for downstream characterization and application. In this work, we highlight a single-step purification of custom sequence- or M13-derived scaffold-based DNA NPs using photocleavable biotin tethers. The process only requires an inexpensive ultraviolet (UV) lamp, and DNA NPs with up to 90% yield and high purity are obtained. We show the versatility of the process in separating two multihelix bundle structures and a wireframe polyhedral architecture.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biotina / ADN de Cadena Simple / Nanopartículas Idioma: En Revista: ACS Appl Mater Interfaces Asunto de la revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biotina / ADN de Cadena Simple / Nanopartículas Idioma: En Revista: ACS Appl Mater Interfaces Asunto de la revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos