Evaluation of Tumorigenic Properties of MDA-MB-231 Cancer Stem Cells Cocultured with Telocytes and Telocyte-Derived Mitochondria Following <i>miR-146a</i> Inhibition.

Babadag, Sena; Altundag-Erdogan, Özlem; Akkaya-Ulum, Yeliz Z; Çelebi-Saltik, Betül

Evaluation of Tumorigenic Properties of MDA-MB-231 Cancer Stem Cells Cocultured with Telocytes and Telocyte-Derived Mitochondria Following miR-146a Inhibition.

Babadag, Sena; Altundag-Erdogan, Özlem; Akkaya-Ulum, Yeliz Z; Çelebi-Saltik, Betül.

Afiliación

Babadag S; Department of Stem Cell Sciences, Hacettepe University Graduate School of Health Sciences, Ankara, Turkey.
Altundag-Erdogan Ö; Center for Stem Cell Research and Development, Hacettepe University, Ankara, Turkey.
Akkaya-Ulum YZ; Department of Stem Cell Sciences, Hacettepe University Graduate School of Health Sciences, Ankara, Turkey.
Çelebi-Saltik B; Center for Stem Cell Research and Development, Hacettepe University, Ankara, Turkey.

DNA Cell Biol ; 43(7): 341-352, 2024 Jul.

Article en En | MEDLINE | ID: mdl-38634821

ABSTRACT

ABSTRACT

Telocytes have some cytoplasmic extensions called telopodes, which are thought to play a role in mitochondrial transfer in intercellular communication. Besides, it is hypothesized that telocytes establish cell membrane-mediated connections with breast cancer cells in coculture and may contribute to the survival of neoplastic cell clusters together with other stromal cells. The aim of this study is to investigate the contribution of telocytes and telocyte-derived mitochondria, which have also been identified in breast tumors, to the tumor development of breast cancer stem cells (CSCs) via miR-146a-5p. The isolation/characterization of telocytes from bone marrow mononuclear cells and the isolation of mitochondria from these cells were performed, respectively. In the next step, CSCs were isolated from the MDA-MB-231 cell line and were characterized. Then, miR-146a-5p expressions of CSCs were inhibited by anti-miR-146a-5p. The epithelial-mesenchymal transition (EMT) was determined by evaluating changes in vimentin protein levels and was evaluated by analyzing BRCA1, P53, SOX2, E-cadherin, and N-cadherin gene expression changes. Our results showed that miR-146a promoted stemness and oncogenic properties in CSCs. EMT (N-cadherin, vimentin, E-cadherin) and tumorigenic markers (BRCA1, P53, SOX2) of CSCs decreased after miR-146a inhibition. Bone marrow-derived telocytes and mitochondria derived from telocytes favored the reduction of CSC aggressiveness following this inhibition.

Asunto(s)

Neoplasias de la Mama; Técnicas de Cocultivo; MicroARNs; Mitocondrias; Células Madre Neoplásicas; Telocitos; Humanos; Telocitos/metabolismo; Telocitos/patología; Células Madre Neoplásicas/patología; Células Madre Neoplásicas/metabolismo; MicroARNs/genética; MicroARNs/metabolismo; Mitocondrias/metabolismo; Mitocondrias/patología; Línea Celular Tumoral; Femenino; Neoplasias de la Mama/patología; Neoplasias de la Mama/metabolismo; Neoplasias de la Mama/genética; Transición Epitelial-Mesenquimal/genética; Regulación Neoplásica de la Expresión Génica; Carcinogénesis/patología; Carcinogénesis/genética; Carcinogénesis/metabolismo

Palabras clave

cancer stem cell; miR-146-5p; microRNA; mitochondria; telocyte

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre Neoplásicas / Neoplasias de la Mama / Técnicas de Cocultivo / MicroARNs / Telocitos / Mitocondrias Límite: Female / Humans Idioma: En Revista: DNA Cell Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Estados Unidos

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