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The effects of a glyphosate-based herbicide on the bovine gametes during an in vitro embryo production model.
Dovolou, E; Nanas, I; Giannoulis, T; Fytsilli, A; Ntemka, A; Anifandis, G; Tsakmakidis, I; Amiridis, G S.
Afiliación
  • Dovolou E; Department of Animal Science, University of Thessaly, Larissa, Greece. Electronic address: entovolou@uth.gr.
  • Nanas I; Department of Obstetrics & Reproduction, Faculty of Veterinary Science, University of Thessaly, Karditsa, Greece.
  • Giannoulis T; Department of Animal Science, University of Thessaly, Larissa, Greece.
  • Fytsilli A; Department of Biochemistry & Biotechnology, Laboratory of Genetics, Comparative and Evolutionary Biology, Larissa, Greece.
  • Ntemka A; Department of Animal Science, University of Thessaly, Larissa, Greece; Clinic of Farm Animals, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece.
  • Anifandis G; Department of Obstetrics and Gynaecology, ART Unit, Faculty of Medicine, University of Thessaly, Larissa, Greece.
  • Tsakmakidis I; Clinic of Farm Animals, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece.
  • Amiridis GS; Department of Obstetrics & Reproduction, Faculty of Veterinary Science, University of Thessaly, Karditsa, Greece.
Environ Pollut ; 350: 123967, 2024 Jun 01.
Article en En | MEDLINE | ID: mdl-38631452
ABSTRACT
Roundup® (R), while it is the most used herbicide globally, and its residues are ubiquitous in urban and suburban areas, its impact on vertebrates' safety remains highly debated. Here, in three in vitro experiments, we investigated the effects of a very low dose (1 ppm) of R on the fertilization capacity and embryo development in cattle. In the first experiment, frozen-thawed bull semen exposed to R for 1 h exhibited reduced motility parameters but unaffected fertilization ability. However, after in vitro fertilization, the rates of embryo formation were significantly lower compared to the untreated controls. In the second experiment, oocytes exposed to R during in vitro maturation showed reduced cleavage rates, and the embryo yield on days 7, 8, and 9 of embryo culture was significantly lower than that of the controls. In the third experiment, oocytes were matured in the presence of R and in a medium containing both R and Zinc, chosen to offer antioxidant protection to the oocytes. Day-7 blastocysts were analyzed for the expression of genes associated with oxidative stress, apoptosis, and epigenetic reprogramming. Exposure to R markedly suppressed embryo formation rates compared to the controls. The combination of R with Zinc restored the blastocyst yield, which on days 8 and 9 was comparable to that of the controls and higher than the groups exposed only to R on all days. The gene expression analysis revealed that R promotes oxidative stress development, triggers apoptosis, and induces epigenetic changes in developing embryos, while zinc presence alleviates these adverse effects of R. These findings imply that even at very low doses, R could be highly toxic, leading to functional abnormalities in both gametes, potentially affecting fertility in both genders.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fertilización In Vitro / Glifosato / Glicina / Herbicidas Límite: Animals Idioma: En Revista: Environ Pollut Asunto de la revista: SAUDE AMBIENTAL Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fertilización In Vitro / Glifosato / Glicina / Herbicidas Límite: Animals Idioma: En Revista: Environ Pollut Asunto de la revista: SAUDE AMBIENTAL Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido