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Down-regulation of wheat Rubisco activase isoforms expression by virus-induced gene silencing.
Perdomo, Juan Alejandro; Scales, Joanna C; Lee, Wing-Sham; Kanyuka, Kostya; Carmo-Silva, Elizabete.
Afiliación
  • Perdomo JA; Lancaster Environment Centre Lancaster University Lancaster UK.
  • Scales JC; School of Pharmacy and Biomedical Sciences University of Central Lancashire Preston UK.
  • Lee WS; Plant Biology and Crop Sciences Rothamsted Research Harpenden UK.
  • Kanyuka K; Biointeractions and Crop Protection Rothamsted Research Harpenden UK.
  • Carmo-Silva E; Biointeractions and Crop Protection Rothamsted Research Harpenden UK.
Plant Direct ; 8(4): e583, 2024 Apr.
Article en En | MEDLINE | ID: mdl-38628621
ABSTRACT
Rubisco activase (Rca) is an essential photosynthetic enzyme that removes inhibitors from the catalytic sites of the carboxylating enzyme Rubisco. In wheat, Rca is composed of one longer 46 kDa α-isoform and two shorter 42 kDa ß-isoforms encoded by the genes TaRca1 and TaRca2. TaRca1 produces a single transcript from which a short 1ß-isoform is expressed, whereas two alternative transcripts are generated from TaRca2 directing expression of either a long 2α-isoform or a short 2ß-isoform. The 2ß isoform is similar but not identical to 1ß. Here, virus-induced gene silencing (VIGS) was used to silence the different TaRca transcripts. Abundance of the transcripts and the respective protein isoforms was then evaluated in the VIGS-treated and control plants. Remarkably, treatment with the construct specifically targeting TaRca1 efficiently decreased expression not only of TaRca1 but also of the two alternative TaRca2 transcripts. Similarly, specific targeting of the TaRca2 transcript encoding a long isoform TaRca2α resulted in silencing of both TaRca2 alternative transcripts. The corresponding protein isoforms decreased in abundance. These findings indicate concomitant down-regulation of TaRca1 and TaRca2 at both transcript and protein levels and may impact the feasibility of altering the relative abundance of Rca isoforms in wheat.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Plant Direct Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Plant Direct Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido